Anti-Inflammatory Response and Muscarinic Cholinergic Regulation during the Laxative Effect of Asparagus cochinchinensis in Loperamide-Induced Constipation of SD Rats

International Journal of Molecular Sciences
Ji-Eun KimDae-Youn Hwang

Abstract

Several types of saponins and herbal plants containing saponins have been reported to have anti-inflammatory or laxative activities. To verify the therapeutic effects of saponin-enriched extracts of Asparagus cochinchinensis (SPA) on the anti-inflammatory response and on the cholinergic regulation in the gastrointestinal system, an alteration on the constipation phenotypes, on the inflammatory responses, and on the muscarinic cholinergic regulation were investigated in the transverse colons of Sprague Dawley (SD) rats with loperamide (Lop)-induced constipation after the treatment of SPA. Significant increases were observed on the total number of stools, the gastrointestinal transit, the thickness of the mucosal layer, the flat luminal surface, the number of paneth cells, and the lipid droplets in the Lop + SPA-treated group as compared to the Lop + Vehicle-treated group. SPA treatment induced the recovery of inflammatory cytokines (TNF-α, IL-1β) and IL-6), inflammatory mediators (NF-κB and iNOS), the total number of infiltered mast cells, and mucin secretion. Also, some similar improvements were observed on the levels of acetylcholine esterase (AChE) activity and on the phosphorylation of myosin light chains (MLC) as well as th...Continue Reading

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Citations

Dec 19, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ji Won ParkDae Youn Hwang
Jun 3, 2021·International Journal of Molecular Sciences·Yun Ju ChoiDae Youn Hwang
Jul 31, 2021·Probiotics and Antimicrobial Proteins·Chih-Ming ChenYing-Chieh Tsai

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Methods Mentioned

BETA
PCR
Extraction
Protein assay
electrophoresis
light microscopy
Assay

Software Mentioned

AlphaView
Leica Application Suite

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