Anti-Influenza Activity of Enterocin B In vitro and Protective Effect of Bacteriocinogenic Enterococcal Probiotic Strain on Influenza Infection in Mouse Model

Probiotics and Antimicrobial Proteins
E I ErmolenkoA N Suvorov

Abstract

In spite of scientific evidence demonstrating the antiviral activity of lactic-acids bacteria, little is known about the mechanism of their action. Previously, several bacteriocins isolated from lactic acid bacteria (LAB) and some other microorganisms were reported as having antiviral activity in vitro. In the present study, chemically synthetized enterocin B (EntB) and the strain E. faecium L3, known as the producer of this peptide, were tested for activity against influenza viruses. The inhibition of cytopathic effect of А/Perth/16/2009(H3N2) and A/South Africa/3626/2013(H1N1) pdm influenza viruses in MDCK cells by chemically synthetized EntB was revealed. The EntB demonstrated antiviral activity at a concentration of 2.5-5 μg/ml depending on the dose of viruses. This peptide exhibited low toxicity in MDCK cells, causing partial damage of the monolayer of the cells only at a concentration above 10 μg/ml. It was also shown, that strain E. faecium L3-protected mice from lethal A/South Africa/3626/2013(H1N1) pdm infection. We speculate that this protective effect of enterococci may be associated with the specific action of enterocin B, which possesses antiviral activity in vitro.

References

Apr 30, 2003·Antiviral Research·Mónica B WachsmanCelia E Coto
May 5, 2005·International Journal of Antimicrobial Agents·Svetoslav D TodorovLeon M T Dicks
Oct 6, 2005·Nature Reviews. Microbiology·Paul D CotterR Paul Ross
Jul 16, 2010·Influenza and Other Respiratory Viruses·Ying-Hen Hsieh
Aug 7, 2010·Food Microbiology·Svetoslav Dimitrov TodorovDjamel Drider
Mar 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Takeshi IchinoheAkiko Iwasaki
May 3, 2013·Probiotics and Antimicrobial Proteins·Nicolás I TorresMichael L Chikindas
Jun 2, 2014·Probiotics and Antimicrobial Proteins·I Al KassaaD Drider
Jan 1, 2013·Bioscience of Microbiota, Food and Health·Alexander Suvorov
Jul 16, 2014·Journal of Virological Methods·Jennifer L DembinskiSiri Mjaaland
Aug 5, 2014·Journal of Applied Microbiology·V M QuintanaM Chikindas
Jan 30, 2016·Genome Announcements·Alena KarasevaAlexander Suvorov
Feb 11, 2016·Applied Microbiology and Biotechnology·Patricia Alvarez-SieiroOscar P Kuipers
May 21, 2016·Frontiers in Microbiology·Empar ChenollJosé A Moreno Muñoz
Aug 3, 2016·Probiotics and Antimicrobial Proteins·Djamel DriderMichael L Chikindas
Aug 9, 2017·Current Opinion in Biotechnology·Michael L ChikindasLeon Mt Dicks
Oct 13, 2017·Cell Host & Microbe·H-Heinrich HoffmannCharles M Rice

❮ Previous
Next ❯

Citations

Sep 26, 2020·Frontiers in Microbiology·Santosh Kumar TiwariMichael L Chikindas
Oct 30, 2020·International Journal of Environmental Research and Public Health·Hassan ZgheibYanath Belguesmia
Feb 4, 2021·Cellular and Molecular Life Sciences : CMLS·Yuxin FuOscar P Kuipers
Mar 31, 2021·Advances in Nutrition·Laurence DaoustAndré Marette
Jul 3, 2021·Marine Drugs·Janira PrichulaMichael S Gilmore
Aug 6, 2021·Current Microbiology·Zhiming LvZeliang Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.