PMID: 7031668Nov 1, 1981Paper

Anti-K562 cell monoclonal antibodies recognize hematopoietic progenitors

Proceedings of the National Academy of Sciences of the United States of America
N S Young, S P Hwang-Chen

Abstract

The K562 leukemia cell has properties of self-renewal and pluripotency similar to those of the hematopoietic stem cell. Monoclonal antibodies to K562 cells have been produced by using hybridoma technology. By radioimmunoassay, some anti-K562 cell antibodies also bind to erythrocyte antigens or peripheral blood mononuclear cells; others are more specific for K562 cells. Antibody binding to hematopoietic progenitors was assayed by using the ability of these cells to form colonies in vitro. After exposure of human bone marrow cells to anti-K562 antibodies and complement, myeloid or erythroid colony formation was inhibited. Some of the inhibitory antibodies showed little binding to mature blood cells by radioimmunoassay, immunofluorescence, and complement cytotoxicity, suggesting that they recognize antigens specific to undifferentiated cells. With the fluorescence-activated cell sorter, one inhibitory antibody was shown to stain only 3% of bone marrow cells. Inhibitory anti-K562 antibodies also bind to myelogenous leukemia cells and virus-transformed lymphocytes. Thus, these antibodies appear to recognize antigens shared by normal hematopoietic progenitors, leukemic cells, and transformed lymphocytes.

References

Aug 1, 1978·The Journal of Experimental Medicine·R J WinchesterH G Kunkel
Jan 1, 1980·Leukemia Research·H P KoefflerD W Golde
Apr 1, 1981·Proceedings of the Society for Experimental Biology and Medicine·B B LozzioA S Feliu

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Citations

Apr 15, 1983·Journal of Immunological Methods·J M Goust, H H Fudenberg
Oct 28, 1986·Biochimica Et Biophysica Acta·C L Reading, Y Takaue
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D FerreroG Rovera
Dec 1, 1985·Immunology Today·G F BurnsJ A Werkmeister
Dec 28, 1983·Biochemical and Biophysical Research Communications·X Z TangK Hall

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