Anti-leishmanial activity of Brevinin 2R and its Lauric acid conjugate type against L. major: In vitro mechanism of actions and in vivo treatment potentials

PLoS Neglected Tropical Diseases
F ZahedifardS Rafati

Abstract

Leishmaniasis, as a major health problem in tropical and sub-tropical areas in the world, needs novel, safe, nontoxic and plausible therapeutic solutions for its control. As a part of innate immune system, natural antimicrobial peptides have a potential to be used as new generation of antibiotics especially after persistent resistance of conventional antimicrobial agents. Brevinin 2R, a member of Defensin families of host defense peptides, showed promising effects against bacterial and fungal infections as well as cancerous cell lines. In the current research, the anti-leishmanial effect of Brevinin 2R and its lauric acid conjugate was investigated against Leishmania major (L. major) parasite. The data revealed that, conjugation of fatty acid to Brevinin 2R, strengthen its effect on L. major promastigotes as well as toxicity and hemolytic effect. These peptides showed anitleishmanial activity through cell membrane disruption and changes in the electrical and mitochondrial membrane potential. No signs of apoptosis induction or caspase activation were detected. Despite its hemolytic and cytotoxic effect in in vitro conditions, lauric acid- Brevinin 2R (L- Brevinin 2R) did not show site specific adverse reactions in animal model. ...Continue Reading

References

Dec 31, 1997·The Journal of Experimental Medicine·R S ChuC V Harding
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y BelkaidD Sacks
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·Keith G NelsonRichard Titus
Jul 15, 2006·Protein Expression and Purification·Marzena Pazgier, Jacek Lubkowski
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Ariane Baratta-MasiniRodrigo Corrêa-Oliveira
Jul 28, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Frédéric FrézardRaul R Ribeiro
Apr 4, 2012·Emerging Infectious Diseases·Sandrine CojeanSophie Matheron
Jun 22, 2012·PLoS Neglected Tropical Diseases·Jair L Siqueira-NetoLucio H Freitas-Junior
Jul 18, 2012·Therapeutics and Clinical Risk Management·Viroj Wiwanitkit
Jul 27, 2012·The Journal of Antimicrobial Chemotherapy·Thomas P C DorloPeter J de Vries
Feb 22, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Suman RijalJean-Claude Dujardin
Jul 10, 2013·BMC Cell Biology·Matthew BrentnallLawrence H Boise
Nov 20, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jenny Campos-SalinasElena Gonzalez-Rey
Apr 8, 2014·The Journal of Biological Chemistry·Jenny Campos-SalinasElena Gonzalez-Rey
Apr 23, 2014·Tropical Parasitology·Sarita Mohapatra
May 9, 2014·PLoS Neglected Tropical Diseases·Juliana Q ReimãoSilvia R B Uliana
May 31, 2015·European Biophysics Journal : EBJ·Shreyasi ThakurMarcelo Nöllmann
Mar 13, 2016·Parasites & Vectors·Corinne LoeuilletMallorie Hide
Jul 15, 2016·Current Topics in Medicinal Chemistry·Katarzyna E Greber, Małgorzata Dawgul
Jul 19, 2016·Nature Reviews. Immunology·Phillip Scott, Fernanda O Novais
Jun 27, 2017·F1000Research·Edoardo Torres-GuerreroRoberto Arenas
Nov 24, 2017·Frontiers in Immunology·Rajiv KumarChristian R Engwerda
Dec 19, 2017·PLoS Neglected Tropical Diseases·Zahra AbdossamadiSima Rafati
Jun 12, 2018·Expert Review of Anti-infective Therapy·Farnaz Zahedifard, Sima Rafati

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Methods Mentioned

BETA
CLIP
Fluorescence
Scanning electron microscopy
PCR
flow cytometry
ELISA

Software Mentioned

Three star
Graph pad prism
Image mining
Flow Jo

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