Anti-mitochondrial antibodies in patients with dilated cardiomyopathy (anti-M7) are directed against flavoenzymes with covalently bound FAD

Clinical and Experimental Immunology
A OttoR Brandsch

Abstract

Anti-mitochondrial antibodies (anti-M7) in sera from patients with dilated cardiomyopathy and myocarditis recognize, besides mitochondrial antigens, bacterial sarcosine dehydrogenase. The common target antigen was identified as the covalently bound FAD of mitochondrial and bacterial flavoenzymes. Thus, anti-M7-positive serum reacted on Western blots exclusively with covalently flavinylated enzymes. The antigenic specificity of anti-M7 sera was reproduced by an antiserum raised in rabbits with 6-hydroxy-D-nicotine oxidase. The heart mitochondrial membrane antigen recognized by anti-M7 serum was identified as the flavoprotein subunit of succinate dehydrogenase, the antigens in rat liver mitochondrial matrix as the flavoenzymes dimethylglycine dehydrogenase and sarcosine dehydrogenase. Anti-M7 serum contained a specific anti-flavoenzyme antibody fraction. Nanomolar concentrations of FAD and riboflavin inhibited the immune reaction on Western blots and in ELISA, and incubation with FAD-agarose depleted the anti-M7 activity of the serum. N-terminally deleted dimethylglycine dehydrogenase proteins were only immunoprecipitated by anti-M7 sera when the FAD was covalently incorporated. An affinity constant (KD) of 10(-8) M was establish...Continue Reading

References

May 1, 1989·Seminars in Liver Disease·P A Berg, R Klein
Oct 1, 1989·Physiological Reviews·D B McCormick
Feb 15, 1987·The Biochemical Journal·M J BarberB A Ackrell
Mar 1, 1968·European Journal of Biochemistry·V D DaiH Sund
Jul 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·A HinkkanenK Decker
Jan 1, 1980·Methods in Enzymology·E Engvall
Mar 15, 1982·Biochemical and Biophysical Research Communications·J G ConboyL E Rosenberg
Sep 15, 1980·Klinische Wochenschrift·P Schuff-Werner, P A Berg
May 24, 1995·Biochimica Et Biophysica Acta·H Baum
Mar 1, 1993·Immunology Today·J S Axford
Apr 1, 1993·Immunology Today·M J Mamula, C A Janeway
Jan 1, 1995·Methods in Enzymology·K M Robinson, B D Lemire
Apr 19, 1996·The Journal of Biological Chemistry·A OttoR Brandsch
Mar 1, 1996·Immunology Today·T R Mosmann, S Sad
May 1, 1997·Seminars in Liver Disease·P A BergM Röcken

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Citations

Apr 16, 2002·Thyroid : Official Journal of the American Thyroid Association·Matthias KasparJack Ronald Wall
Aug 9, 2002·European Journal of Heart Failure·Alida L P CaforioWilliam J McKenna
Dec 21, 2000·Clinical and Experimental Immunology·G CicekR Brandsch
May 15, 2009·The FEBS Journal·Dominic P H M HeutsMarco W Fraaije
Apr 3, 2008·Current Opinion in Cardiology·Alida L P Caforio, Sabino Iliceto
Sep 11, 2008·Autoimmunity·Alida L P CaforioSabino Iliceto
Jan 19, 2021·Internal Medicine·Tetsuro YokokawaYasuchika Takeishi
Feb 20, 2021·Apoptosis : an International Journal on Programmed Cell Death·Jing ZhangWei Ding
Apr 22, 2008·International Journal of Biological Macromolecules·Carmen BrizioMaria Barile

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