Anti-mycobacterial triterpenes from the Canadian medicinal plant Alnus incana

Journal of Ethnopharmacology
Haoxin LiChristopher A Gray

Abstract

Alnus incana, commonly known as the gray or speckled alder, is a medicinal plant used by some Canadian First Nations to treat symptoms associated with tuberculosis. The aim of this study was to assess the anti-mycobacterial activity of an Alnus incana bark extract and to identify the active constituents of the extract. Methanolic extracts of the bark of A. incana were subjected to bioassay guided fractionation using Mycobacterium tuberculosis (H37Ra). The active constituents were identified by NMR and MS. Four pentacyclic lupane triterpenes were isolated and were identified as betulin, betulinic acid, betulone and lupenone. Betulin displayed a MIC of 12.5 μg/mL and an IC50 of 2.4 μg/mL against M. tuberculosis (H37Ra). Betulinic acid and betulone showed lower anti-mycobacterial activities with IC50 values of 84 and 57 μg/mL respectively. Lupenone was inactive against M. tuberculosis (H37Ra). Betulin, betulinic acid and betulone were identified as the major anti-mycobacterial constituents in the bark of A. incana and the functionality at carbons 3 and 28 of the lupane skeleton would seem to be important in determining the anti-mycobacterial activity of the triterpenes. This work supports the ethnopharmacological use of A. incana ...Continue Reading

References

Dec 1, 1994·Journal of Ethnopharmacology·A R McCutcheonG H Towers
Dec 1, 1995·Planta medica·H MatsuuraG H Towers
Dec 1, 1995·Journal of Ethnopharmacology·A R McCutcheonG H Towers
Jan 1, 1996·Journal of Natural Products·G SaxenaG H Towers
Aug 1, 1997·Planta medica·Y H KuoK H Lee
Apr 19, 2002·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·G A WächterB N Timmermann
May 7, 2002·Natural Product Letters·Joseph K Rugutt, Kipngeno J Rugutt
Jul 18, 2002·Journal of Asian Natural Products Research·Dong-Sheng MingDe-Quan Yu
Apr 28, 2004·Phytochemistry·Charles MutaiVassilios Roussis
Jul 2, 2004·Planta medica·Jian-Qiao GuBarbara N Timmermann
Jul 21, 2004·Fitoterapia·A T NguyenJ Fontaine
Apr 7, 2006·Archives of Pharmacal Research·Sang Zin ChoiKang Ro Lee
Nov 23, 2006·Natural Product Reports·Pavel A Krasutsky
May 22, 2007·International Journal of Food Sciences and Nutrition·Ocksook YiDongwuk Cho
Dec 11, 2007·Canadian Journal of Physiology and Pharmacology·Eduardo M JovelG H Neil Towers
Oct 24, 2008·Journal of Mass Spectrometry : JMS·B Rhourri-FrihM Lafosse
Jan 12, 2012·Journal of Ethnopharmacology·Haoxin LiChristopher A Gray
Mar 27, 2012·The Journal of Infectious Diseases·Christian LienhardtJan Gheuens
Aug 11, 2012·Journal of Ethnopharmacology·Caitlyn D CarpenterChristopher A Gray
Mar 19, 2013·Journal of Ethnopharmacology·Taryn O'NeillChristopher A Gray

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Citations

Dec 24, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ionuţ LedeţiCristina Dehelean
May 9, 2016·European Journal of Drug Metabolism and Pharmacokinetics·Olga N PozharitskayaJessica M Rosenholm
May 18, 2017·Natural Product Research·Weijie Li, Yeyu Xiao
Apr 4, 2017·Journal of Natural Products·Miroslav NovakovicSlobodan Milosavljevic
Aug 22, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xueyang RenGaimei She
Oct 24, 2019·Analytical and Bioanalytical Chemistry·Riikka-Marjaana RäsänenTiina J Kauppila
Jul 7, 2021·Natural Product Reports·Jianying HanYunjiang Feng

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