Anti-Neuronal Antibodies Within the IVIg Preparations: Importance in Clinical Practice

Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics
Maria M DimitriadouMarinos C Dalakas

Abstract

Our study objective was testing for anti-neuronal autoantibodies within commercially available intravenous immunoglobulin (IVIg) preparations. Sixteen samples from 5 different commercially available IVIg preparations were tested with cell-based assays (CBA) and enzyme-linked immunosorbent assay (ELISA) to detect and characterize common neuronal autoantibodies, and with immunohistochemistry on teased fibers from mouse sciatic nerve and on mouse brain sections to screen for nodal and not yet identified neuronal antigens. In 15/16 IVIg preparations, anti-GAD antibodies were detected in titers ranging from 40 to 1507 IU/mL, as typically seen in type 1 diabetes, but not in the range (> 2000 IU/mL) seen in GAD-positive neurological patients. None of the preparations was however positive with anti-GAD CBA. Antibodies to AQP4 were also detected by ELISA in 15/16 IVIg preparations with titers comparable to those seen in AQP4-seropositive NMO patients; with CBA, however, all IVIg samples were AQP4-negative. IVIg preparations contained IgG-anti-MAG antibodies by ELISA at statistically significant higher titers compared to controls. Two of the 16 IVIg samples were positive for human 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) a...Continue Reading

References

Oct 1, 1996·Acta Medica Okayama·S NishimuraK Hashimoto
May 1, 1997·Annals of Internal Medicine·Marinos C Dalakas
Oct 6, 1998·Journal of Immunological Methods·S Lacroix-DesmazesM Kazatchkine
Sep 8, 2001·The New England Journal of Medicine·M Kazatchkine, S Kaveri
May 20, 2004·JAMA : the Journal of the American Medical Association·Marinos C Dalakas
Jun 19, 2008·Autoimmunity Reviews·Janakiraman VaniS Kaveri
Feb 22, 2011·Journal of Autoimmunity·Eleni I KampylafkaAthanasios G Tzioufas
May 16, 2013·Journal of Neuroimmunology·Haris AlexopoulosAthanasios G Tzioufas
Dec 19, 2013·Brain Pathology·Monika Bradl, Hans Lassmann
Jan 7, 2015·Nature Reviews. Neurology·Jan D LünemannMarinos C Dalakas
Jan 28, 2015·Nature Reviews. Neurology·Panos StathopoulosMarinos C Dalakas
Jul 4, 2017·The Journal of Allergy and Clinical Immunology. in Practice·Tukisa D Smith, Charlotte Cunningham-Rundles
Feb 7, 2018·Therapeutic Advances in Neurological Disorders·Marinos C Dalakas
Jun 29, 2018·Frontiers in Immunology·Yi GuoZheng Xiao

Citations

Apr 16, 2021·Therapeutic Advances in Neurological Disorders·Popianna TsiortouMarinos C Dalakas
Sep 10, 2020·Neurología : publicación oficial de la Sociedad Española de Neurología·E Fernández DíazT Segura

Methods Mentioned

BETA
Assay
ELISA

Related Concepts

Epitopes
Autoantibodies
Autoimmune Diseases
Brain
Diabetes Mellitus, Insulin-Dependent
Enzyme-Linked Immunosorbent Assay
Immunoglobulin G
Immunohistochemistry
Laboratory mice
Structure of Sciatic Nerve

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Antibody Repertoire Diversity

Antibody repertoire diversity and its role during natural infection is a prerequisite for molecular and structural elucidation of functionally protective immunity. Discover the latest insights into antibody diversity here.

Anti-N-Methyl-D-Aspartate Receptor Encephalitis

Anti-N-Methyl-D-Aspartate (NMDA) receptor encephalitis is a form of of brain inflammation due to antibodies against NMDA receptors. Discover the latest research on anti-NMDA receptor encephalitis here.