Anti-osteoclastogenic effect of epigallocatechin gallate-functionalized gold nanoparticles in vitro and in vivo

International Journal of Nanomedicine
Shenting ZhuBin Peng

Abstract

Background: Epigallocatechin gallate (EGCG), the major anti-inflammatory compound in green tea, has been shown to suppress osteoclast (OC) differentiation. However, the low aqueous solubility of EGCG always leads to poor bioavailability, adverse effects, and several drawbacks for clinical applications. Purpose: In this study, we synthesized EGCG-capped gold nanoparticles (EGCG-GNPs) to solve the drawbacks for clinical uses of EGCG in bone destruction disorders by direct reduction of HAuCl4 in EGCG aqueous solution. Methods and Results: The obtained EGCG-GNPs were negatively charged and spherical. Theoretical calculation results suggested that EGCG was released from GNPs in an acidic environment. Cellular uptake study showed an obviously large amount of intracellular EGCG-GNPs without cytotoxicity. EGCG-GNPs exhibited better effects in reducing intracellular reactive oxygen species levels than free EGCG. A more dramatic anti-osteoclastogenic effect induced by EGCG-GNPs than free EGCG was observed in lipopolysaccharide (LPS)-stimulated bone marrow macrophages, including decreased formation of TRAP-positive multinuclear cells and actin rings. Meanwhile, EGCG-GNPs not only suppressed the mRNA expression of genetic markers of OC dif...Continue Reading

Citations

May 8, 2020·Journal of Biomedical Materials Research. Part a·Shengnan LiaoYili Qu
Nov 19, 2020·Anais Da Academia Brasileira De Ciências·Shady M MostafaAbdelgawad A Fahmi

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Methods Mentioned

BETA
dynamic light-scattering
transmission electron microscopy
PCR
dynamic
surface plasmon resonance
dynamic light scattering
X-ray
nuclear translocation

Software Mentioned

SPSS
Materials
COSMO
DMol3
Studio

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