Anti-Oxidant and Anti-Apoptotic Effects of Berberine in Pentylenetetrazole-Induced Kindling Model in Rat

Journal of Epilepsy Research
Vaishali GunaAmitava Chakrabarti

Abstract

Berberine (BBR) is derived from the Berberis species and has demonstrated beneficial effects in various neurodegenerative disorders in animal models. The objective of this study was to evaluate the antiepileptic, antioxidative, and anti-apoptotic effects of BBR in a pentylenetetrazole (PTZ)-induced kindling model of epilepsy in rats. A total of 30 male Wistar rats were randomly assigned to receive BBR (100 mg/kg, oral), sodium valproate (200 mg/kg, i.p.), or saline (0.9% NaCl, i.p.) followed by PTZ (35 mg/kg, i.p.) on alternate days until the animal developed kindling or for 10 weeks. Histopathological examination of the hippocampus; DNA fragmentation study; tests for malondialdehyde, superoxide dismutase, glutathione peroxidase, and reduced glutathione; and gene expression studies (nrf2, bcl-2, bax, and caspase 3) were conducted on whole brain tissue after 10 weeks or kindling. The percentage of kindled animals, histopathological score, malondialdehyde level, and caspase 3 gene expression were significantly lower in the BBR group than in the PTZ group. Superoxide dismutase levels, reduced glutathione levels, and bcl-2 gene expression were significantly higher in the BBR group than in the PTZ group. The present study demonstrat...Continue Reading

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Mar 1, 1972·Electroencephalography and Clinical Neurophysiology·R J Racine
Aug 10, 2000·European Journal of Biochemistry·J B SchulzJ Dichgans
Jan 25, 2003·Pharmacology, Biochemistry, and Behavior·Y K GuptaA K Srivastava
Mar 31, 2004·The Journal of Thoracic and Cardiovascular Surgery·Richard J MyungWilliam M DeCampli
Jul 20, 2010·Epilepsy & Behavior : E&B·Pravinkumar BhutadaDharmendra Mundhada
Jan 25, 2011·Lancet Neurology·Asla Pitkänen, Katarzyna Lukasiuk
Apr 4, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Ya-Yun HsuYi-Ching Lo
Apr 24, 2012·Neuropharmacology·Annamaria VezzaniRaymond J Dingledine
Oct 16, 2013·Biological Chemistry·Jérôme LugrinLucas Liaudet

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Citations

Jan 19, 2021·Life Sciences·Fatma H ShakerHala O El-Mesallamy
Dec 29, 2020·Epilepsy & Behavior : E&B·Priya MishraSatyendra Kumar Rajput
Feb 23, 2020·Current Molecular Pharmacology·Vipin K VermaJagriti Bhatia
Nov 9, 2021·Journal of Biochemical and Molecular Toxicology·Fatemeh B TabriziGholamreza Karimi

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Methods Mentioned

BETA
MDA
electrophoresis
PCR

Software Mentioned

Statistical Package for the Social Sciences

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