PMID: 8963031Sep 1, 1995Paper

Anti-peptide monoclonal antibodies to intestinal mucin 3

Journal of Gastroenterology and Hepatology
V ApostolopoulosI F McKenzie

Abstract

Second generation anti-peptide monoclonal antibodies were produced using synthetic peptide SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS) as an antigen which contains two of the MUC3 tandem repeats. Three monoclonal antibodies were produced (M3.1, M3.2 and M3.3) which reacted with the immunizing peptide, but also reacted strongly with colon tissues. Using the immunoperoxidase staining technique, two of the monoclonal antibodies, M3.1 and M3.2, reacted with both colon carcinoma and normal colon tissue whereas the antibody M3.3 reacted with normal colon tissues but very weakly, if not at all, with colon carcinoma. MUC3 was found to be distributed in colon and rectum, and was also present to a lesser extent in breast, lung and salivary gland tissues. Analysis of mucin molecules by Western blotting, revealed the antigen detected by the antibodies (M3.1 and M3.2) to be of a high relative molecular mass. M3.1 and M3.3 reacted with eptiopes SITTTE and PSFTSS, respectively. M3.2 did not react with any of the 6-mer peptides, even though it reacted with the full length VNTR (2 repeat peptide--SIB35). Anti-MUC3 peptide antibodies appear to react with colon, rectum, breast, salivary gland and lung tissues, and represent a new method of produ...Continue Reading

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Citations

Apr 1, 1999·American Journal of Respiratory Cell and Molecular Biology·S H BernackiS H Randell
Feb 7, 2012·Cancer Letters·Shikha TarangSurinder K Batra
Feb 21, 2006·Biochimica Et Biophysica Acta·Mahefatiana AndrianifahananaSurinder K Batra
Dec 25, 2016·Histochemistry and Cell Biology·Anthony Corfield
Nov 14, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Y CaoU Karsten
Jul 3, 1998·International Journal of Cancer. Journal International Du Cancer·C de BolòsF X Real
Jan 1, 2008·Arthritis and Rheumatism·Michael V VolinAlisa E Koch
Jun 17, 1998·International Journal of Cancer. Journal International Du Cancer·P X XingI F McKenzie
Jul 16, 2008·Journal of Proteome Research·Nicole L WilsonNiclas G Karlsson

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