Anti-peptidylarginine deiminase-4 antibodies at mucosal sites can activate peptidylarginine deiminase-4 enzyme activity in rheumatoid arthritis.

Arthritis Research & Therapy
M Kristen DemoruelleErika Darrah

Abstract

Mucosal sites are hypothesized to play a role in the development of rheumatoid arthritis (RA). Since serum anti-peptidylarginine deiminase (PAD)4 antibodies, including a subset that cross-react with PAD3 (PAD3/4), are specific for RA and associate with severe disease, we sought to examine whether anti-PAD4 and anti-PAD3/4 antibodies were present in the lung and oral mucosa of subjects with RA and "at-risk" for RA. We included 37 RA, 25 healthy control, and 46 subjects "at-risk" for RA based on familial RA and/or serum anti-citrullinated protein antibody (ACPA) positivity. Paired serum, sputum, and saliva were evaluated for anti-PAD4 and anti-PAD3/4 using immunoprecipitation and ACPA using ELISA. Immunoglobulins (Ig) were purified from representative samples, and their effect on citrullination of histone H3 by recombinant human PAD4 was measured by anti-citH3 immunoblot. Anti-PAD4 antibodies were detected in the serum of 6/37 (16.2%), sputum of 3/37 (8.1%), and saliva of 3/33 (9.1%) RA subjects and in the serum and sputum of 1/46 (2.2%) at-risk subjects. None of the healthy controls had anti-PAD4 antibodies at any site. Serum, sputum, and salivary anti-PAD4 antibodies were more prevalent in RA subjects with RA duration >2 years....Continue Reading

References

May 25, 2010·Arthritis and Rheumatism·Jason R KolfenbachV Michael Holers
Nov 20, 2016·Microbiome·Jose U ScherAnca I Catrina
Dec 10, 2017·Best Practice & Research. Clinical Rheumatology·Vijay JoshuaAnca I Catrina
Aug 17, 2018·Nature Reviews. Rheumatology·V Michael HolersKevin D Deane
Oct 10, 2018·Annals of the Rheumatic Diseases·Laura Martinez-PratThierry Dervieux
Jan 20, 2019·Arthritis Research & Therapy·Beatriz Rodríguez-LozanoFederico Díaz-González

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Citations

Sep 4, 2021·Immunology·Utsa Karmakar, Sonja Vermeren

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Software Mentioned

GraphPad Prism
SPSS

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