Anti-peripherin B lymphocytes are positively selected during diabetogenesis

Molecular Immunology
Jorge CarrilloJoan Verdaguer

Abstract

Rearrangement analysis of immunoglobulin genes is an exceptional opportunity to look back at the B lymphocyte differentiation during ontogeny and the subsequent immune response, and thus to study the selective pressures involved in autoimmune disorders. In a recent study to characterize the antigenic specificity of B lymphocytes during T1D progression, we generated hybridomas of islet-infiltrating B lymphocytes from NOD mice and other related strains developing insulitis, but with different degrees of susceptibility to T1D. We found that a sizable proportion of hybridomas produced monoclonal antibodies reactive to peripherin, an intermediate filament protein mainly found in the peripheral nervous system. Moreover, we found that anti-peripherin antibody-producing hybridomas originated from B lymphocytes that had undergone immunoglobulin class switch recombination, a characteristic of secondary immune response. Therefore, in the present study we performed immunoglobulin VL and VH analysis of these hybridomas to ascertain whether they were derived from B lymphocytes that had undergone antigen-driven selection. The results indicated that whereas some anti-peripherin hybridomas showed signs of oligoclonality, somatic hypermutation a...Continue Reading

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Citations

Nov 6, 2010·Current Opinion in Immunology·F Susan WongLi Wen
Jan 12, 2010·Journal of Autoimmunity·Jayne L ChamberlainVanda A Lennon
Dec 8, 2015·European Journal of Immunology·Jorge CarrascalJoan Verdaguer
Mar 13, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nahir GarabatosThomas Stratmann
Feb 18, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eva Codina-BusquetaMercè Martí
Sep 18, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeremy J RacineDavid V Serreze
May 11, 2021·Frontiers in Immunology·Manuel Sáez MoyaPablo Engel

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