Anti-phosphatidylcholine antibodies in patients with brucellosis

Journal of Medical Microbiology
M A CasaoC Gamazo

Abstract

An indirect ELISA was adapted to measure individual classes of anti-phosphatidylcholine (PC) antibodies in patients with brucellosis; a comparison was made with patients with other infectious diseases and healthy human controls. Immunoconjugates of alpha, gamma or mu chain specificity were used. The results were compared with those of conventional tests for brucella antibodies, Rose Bengal, microagglutination (MAT) and Coombs tests, as well as with VDRL. The results indicated that although all sera from patients with brucellosis were VDRL negative, 97% of them (30 of 31) had higher levels of IgA or IgG anti-PC antibodies than the healthy control group. The IgM anti-PC antibodies isotype was not discriminatory between both groups. Seven sera (19%) from patients with other infections had values of IgG anti-PC higher than those of the healthy group; three of them were VDRL positive. The mean ELISA values of IgG and IgA anti-PC (serum diluted 1 in 1600) in the brucellosis and healthy groups were: IgG, 0.80 SEM 0.17 versus 0.23 SEM 0.10; and IgA, 0.86 SEM 0.26 versus 0.35 SEM 0.15. The sensitivity and specificity for the ELISA-PC were: IgG, 96.8% and 96.5%, respectively; IgA, 90.3% and 89.7%, respectively. Only one of 14 individuals...Continue Reading

Citations

Jan 28, 2003·Progress in Lipid Research·Christian SohlenkampOtto Geiger
Mar 18, 1999·Molecular and Biochemical Parasitology·M H Villas BôasE B Bergter
Feb 18, 2006·Journal of Bacteriology·Diego J ComerciRodolfo A Ugalde
Mar 23, 2002·Journal of Autoimmunity·Javier CabiedesDonato Alarcón-Segovia
Oct 20, 2019·Biomedical Journal·Simon Fillatreau

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