Anti-proliferation effects of Twist gene silencing in gastric cancer SGC7901 cells

World Journal of Gastroenterology : WJG
Hui ZhangHong-Yi Liu

Abstract

To study the role of Twist gene in gastric cancer by gene silencing, including the potential of induction of apoptosis, cell cycle arrest, and proliferation inhibition in human malignant gastric SGC7901 cells. The expression level of Twist in gastric cancer samples was measured by immunohistochemistry. The effects of Twist gene silencing were detected at both mRNA and protein levels by RT-PCR and Western blot. We also evaluated the cell proliferation and apoptosis by CCK-8 assay and flow cytometry. We determined the activity of caspase-3 and caspase-9 with a caspase activity assay kit. Cell cycle distribution was analyzed by flow cytometry. Cell migration and invasion ability was evaluated by wound scratch assay and Boyden chamber assay. Twist protein was highly expressed in gastric cancer samples. Twist gene silencing significantly induced apoptosis, cell cycle arrest at G0/G1 phase, proliferation inhibition, and reduced the ability of migration and invasion in human gastric cancer SGC7901 cells. Meanwhile, both caspase-3 and caspase-9 were activated. The Twist gene could serve as a potential molecular target for gene therapy of gastric cancer with targeted small interfering RNA.

References

Aug 13, 1999·Genes & Development·A GrossS J Korsmeyer
Sep 15, 1999·Genes & Development·R MaestroG J Hannon
May 10, 2001·Current Opinion in Cell Biology·K M RyanK H Vousden
May 30, 2003·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·David M Roder
May 3, 2005·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Wataru YasuiHirofumi Nakayama
Jun 17, 2005·Cancer Research·Wai Kei KwokXianghong Wang
Jun 2, 2006·Cancer Genetics and Cytogenetics·Farhad VesunaVenu Raman
Jan 20, 2007·International Journal of Cancer. Journal International Du Cancer·Kenoki OhuchidaMasao Tanaka
Mar 24, 2007·Nature Reviews. Molecular Cell Biology·Karen H Vousden, David P Lane
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Jul 22, 2009·Journal of Visualized Experiments : JoVE·Jeff Partridge, Paula Flaherty
Apr 20, 2009·Zhongguo fei ai za zhi = Chinese journal of lung cancer·Linping HuiZeshi Cui
Jul 19, 2011·Developmental Cell·Jean-Claude Martinou, Richard J Youle
Apr 3, 2012·World Journal of Gastroenterology : WJG·Jing-Jing JingYuan Yuan
Jul 23, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Md Asaduzzaman KhanJunjiang Fu
Aug 13, 2013·Experimental and Therapeutic Medicine·Min ZhaoZheng-Sheng Wu

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Citations

Oct 7, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chae Hwa KwonDo Youn Park

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