Anti-proliferative and cytoskeleton-disruptive effects of icariin on HepG2 cells

Molecular Medicine Reports
Zhi-Min WangYan-Ling Ren

Abstract

Several biological properties of icariin have been identified, including its anticancer effect. However, the potential mechanisms underlying the effect of icariin on HepG2 hepatocellular carcinoma cells remain to be elucidated. The aim of the present study was to examine the effects of icariin on the proliferation and cytoskeleton of HepG2 cells. A 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5 diphenyltetrazolium bromide assay was used to assess the antiproliferative effects of icariin and to determine the optimal concentration and treatment schedule of icariin on the HepG2 cells. Cell cycle analysis was performed using fluorescence activated cell sorting, the protein expression of B‑cell lymphoma (Bcl)‑2 was determined using immunohistochemical and western blot analyses, and F‑actin in the cells was examined using confocal microscopy. The chemotherapeutic drug, oxaliplatin, was used as a positive control. The results demonstrated that the optimal concentration of icarrin to produce an antiproliferative effect on HepG2 cells was 10‑5 mol/l, and the optimal treatment duration was 72 h. The icariin group had a significantly higher proportion of cells in the G0/G1 phase, compared with the control group, treated with high glucose Dulbecco's mod...Continue Reading

References

May 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K Okuda
Feb 7, 1998·Science·A Hall
Sep 12, 1998·Science·J M Adams, S Cory
Jun 25, 2002·Nature Cell Biology·Emanuela ColomboPier Giuseppe Pelicci
Apr 11, 2003·Molecular Biology of the Cell·Ekaterina V ShumilinaSofia Yu Khaitlina
Dec 10, 2003·The Lancet Oncology·Wei Hu, John J Kavanagh
May 27, 2004·World Journal of Gastroenterology : WJG·Xia HuoZhong-Xian Piao
May 8, 2007·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·D-W ZhangX-S Yao
Oct 11, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Huai-Chia ChangRuei-Ming Chen
Nov 26, 2009·The American Journal of Chinese Medicine·Jin-Xia YangXue-Mei Wang
Jun 10, 2010·Clinical Journal of Oncology Nursing·Rose M Bell
Jul 23, 2011·Chinese Medicine·Wen TanYitao Wang
Sep 20, 2011·European Journal of Pharmacology·Luigi FiumeGiuseppina Di Stefano
Nov 22, 2011·Chinese Journal of Cancer·Qing-Qing WangJing-Ping Yun
Mar 30, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Ming-Der ShiLi-Yu Tsai
Sep 11, 2013·Biochemistry·Ertan OzyamakArash Komeili

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.