Anti-proliferative efficacy of icariin on HepG2 hepatoma and its possible mechanism of action

The American Journal of Chinese Medicine
Jin-xia YangXue-mei Wang

Abstract

The aim of the present work was to explore the anti-hepatoma effects of icariin both in vitro and in vivo and to elucidate its potential mechanism of action. The MTT assay was applied to test the anti-proliferative effects of icariin in vitro. HepG2 bearing NMRI nu/nu mice were used to test the anticancer effects of icariin in vivo. Immunohistochemical assay and flow cytometry assay (FACS) were applied to detect the possible mechanisms of action of icariin. MTT assay illustrated that icariin inhibited the proliferation of HepG2 cells in a concentration dependent manner; meanwhile, icariin inhibited the tumor growth in HepG2 bearing NMRI nu/nu mice. The tumor weight was inhibited by 55.6% and tumor volume was inhibited by 47.2%. Icariin did not influence the spleen and body weights or blood parameters. Immunohistochemical analysis indicated that the expressions of both CD31 and Ki67 in the icariin treated group were significantly lower than those in the control group (p < 0.01). FACS assay showed that icariin dramatically decreased the percentage of CD4+ and CD8+ cells in bone marrow and CD19+ cells in blood on day 8. On day 17, the percentage of CD8+ cells in blood was lower than those in the control group. CD4/CD8 ratio in ica...Continue Reading

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Citations

Sep 4, 2015·Molecular Medicine Reports·Zhi-Min WangYan-Ling Ren
Apr 11, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Chao Qu, Zhen Chen
Aug 6, 2015·The American Journal of Chinese Medicine·Xiao-Jia ChenYi-Tao Wang
Jul 23, 2016·Frontiers in Pharmacology·Hui-Li TanBey-Hing Goh
Jun 22, 2017·International Journal of Biological Macromolecules·Weixi ShenLi Zhong
Nov 13, 2017·Pharmacological Reports : PR·Hong ZhangZhi Wang
Feb 11, 2015·Cell Biochemistry and Biophysics·Minghe ZhaiHongmei Zhao
Jun 17, 2020·Chemical Society Reviews·Saeid KargozarMasoud Mozafari
Apr 24, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Estefanny Ruiz GarcíaReggiani Vilela Gonçalves

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