Anti-pseudomonal and anti-endotoxic effects of surfactin-stabilized biogenic silver nanocubes ameliorated wound repair in streptozotocin-induced diabetic mice

Artificial Cells, Nanomedicine, and Biotechnology
Natarajan KrishnanRajesh Kannan Velu

Abstract

Pseudomonas aeruginosa and its lipopolysaccharides play a key role in the pathogenesis of diabetic foot infection, for which, currently no effective therapeutic agents are available. Hence, newer forms of therapeutic agents are required for treating Pseudomonas aeruginosa infection. In this present study, nanocrystalline silver nanoparticles (AgNPs) were synthesized using culture filtrate of Brevibacillus brevis KN8(2) followed by an investigation of its in vivo anti-pseudomonal and anti-endotoxic properties. Biosynthesized AgNPs was predominantly cubical in shape with an average particle size of 15.40 nm as observed through field emission scanning electron microscopy (FESEM) and X-ray diffraction analysis. The LC-ESI-MS/MS analysis indicates the presence of surfactin in culture filtrate of B. brevis KN8(2). The MIC of surfactin-stabilized AgNPs against P. aeruginosa was 10 μg ml-1 and its wound repair activity was observed in P. aeruginosa-infected wounds of diabetic mice by measuring wound area closure, bacterial counts, mRNA expressions, and histopathology. Further, surfactin-stabilized AgNPs suppressed the transcription of LPS-triggered expression of the TNF-α in wounds that LPS-assisted extension of wound repair in diabete...Continue Reading

References

Nov 27, 1999·FEMS Immunology and Medical Microbiology·B A Lipsky
Dec 7, 2000·Photochemistry and Photobiology·N KömerikS Poole
Mar 17, 2004·Current Opinion in Infectious Diseases·Ruth Edwards, Keith G Harding
Nov 8, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Benjamin A LipskyMichael Zasloff
May 1, 2009·ChemMedChem·Kenneth K Y WongPaul K H Tam
May 19, 2009·Nanomedicine : Nanotechnology, Biology, and Medicine·Amanulla Mohammed FayazRamasamy Venketesan
Jul 25, 2009·The Journal of Investigative Dermatology·Saher HamedLuc Teot
Aug 8, 2009·Colloids and Surfaces. B, Biointerfaces·M M Ganesh Babu, P Gunasekaran
Jun 29, 2010·International Journal of Antimicrobial Agents·Swarna JaiswalPatrick McHale
Dec 18, 2010·Canadian Journal of Microbiology·Kandasamy KathiresanSaravanakumar Kandasamy
May 4, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·S KaviyaK Srinivasan
Jan 11, 2012·Clinical Microbiology Reviews·Brian M PetersMark E Shirtliff
Jun 29, 2012·Journal of Diabetes and Its Complications·Haloom RafehiTom C Karagiannis
Jul 5, 2012·Current Diabetes Reports·Robert V Intine, Michael P Sarras
Sep 26, 2012·Medical Microbiology and Immunology·Chase WattersKendra P Rumbaugh
Jun 19, 2013·The International Journal of Lower Extremity Wounds·Markus W LöfflerStefan Beckert
Oct 16, 2013·Infection and Immunity·Chase WattersKendra P Rumbaugh
Dec 15, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Chidambaram Kulandaisamy VenilWan Azlina Ahmad
Feb 26, 2016·Nanoscale Research Letters·Khwaja Salahuddin Siddiqi, Azamal Husen

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Citations

Jan 8, 2019·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Vanessa Santana Vieira SantosBoscolli Barbosa Pereira
Jan 28, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Abdul AhadAbdullah M Al-Mohizea
Apr 23, 2020·Advances in Wound Care·Barbara Blanco-FernandezSoledad Pérez-Amodio
Oct 13, 2020·Biotechnology and Applied Biochemistry·Hosna ZareMojtaba Sankian
Jun 26, 2021·Critical Reviews in Biotechnology·Marcia Nitschke, Crisiane Aparecida Marangon
Nov 27, 2021·Advanced Science·Yuntao LiuGuoqing Pan

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