Anti-Restriction Protein, KlcAHS , Promotes Dissemination of Carbapenem Resistance

Frontiers in Cellular and Infection Microbiology
Wei LiangYuan Lu

Abstract

Carbapenemase-producing Klebsiella pneumoniae (KPC) has emerged and spread throughout the world. A retrospective analysis was performed on carbapenem-resistant K. pneumoniae isolated at our teaching hospital during the period 2009-2010, when the initial outbreak occurred. To determine the mechanism(s) that underlies the increased infectivity exhibited by KPC, Multilocus Sequence Typing (MLST) was conducted. A series of plasmids was also extracted, sequenced and analyzed. Concurrently, the complete sequences of blaKPC-2-harboring plasmids deposited in GenBank were summarized and aligned. The blaKPC-2 and KlcA HS genes in the carbapenem-resistant K. pneumoniae isolates were examined. E. coli strains, carrying different Type I Restriction and Modification (RM) systems, were selected to study the interaction between RM systems, anti-RM systems and horizontal gene transfer (HGT). The ST11 clone predominated among 102 carbapenem-resistant K. pneumoniae isolates, all harbored the blaKPC-2 gene; 98% contained the KlcA HS gene. KlcA HS was one of the core genes in the backbone region of most blaKPC-2 carrying plasmids. Type I RM systems in the host bacteria reduced the rate of pHS10842 plasmid transformation by 30- to 40-fold. Presen...Continue Reading

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Datasets Mentioned

BETA
KF724507.1
KF826293.1
KF724506.1
KF826292.1
KC609322

Methods Mentioned

BETA
PCR

Software Mentioned

SPSS
WHONET
Snap gene viewer
Blast Ring Image Generator
Primer Premier

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