PMID: 9442918Jan 27, 1998Paper

Anti-ROS-DNA monoclonal antibody as molecular probe for oxidative DNA damage

Biochemistry and Molecular Biology International
B T AshokR Ali

Abstract

Modification of 400 bp (approximate size) calf thymus DNA with OH radical resulted in lowered Tm, modification of thymine (58.3%), guanine (20%) and single strand breaks. Monoclonal antibodies (mAb) generated against ROS-DNA were of IgG1 subclass. The mAb showed strong binding to ROS-DNA and ROS-modified bases and polymers, in particular, of thymine. The mAb, therefore, preferentially recognizes ROS-modified epitopes on nucleic acids. Distinct binding to DNA isolated from aged, but not from normal humans by the monoclonal antibody was observed. The antibody effectively recognized oxidative lesions in DNA from cancer patients. These studies demonstrate the potential application of the mAb as an immunochemical probe to detect oxidative DNA lesions.

Citations

Jun 20, 2003·Experimental Gerontology·Badithe T Ashok, Rashid Ali
Aug 5, 1999·Experimental Gerontology·B T Ashok, R Ali
Feb 28, 2003·Clinical and Experimental Immunology·H AhsanR Ali
Apr 24, 2003·Experimental Biology and Medicine·Francesco MazzaNader G Abraham

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