Anti-serum with anti-autoantibody activity decreases autoantibody-positive B lymphocytes and type 1 diabetes of female NOD mice

Autoimmunity
Lijuan LuanSifeng Chen

Abstract

Type 1 diabetes mellitus (T1DM) is an autoimmune disease characterized by an autoimmune-mediated loss of insulin secreting β-cells. Each B lymphocyte clone that escapes immune tolerance produces a specific antibody. No specific treatment against autoantibodies is available for autoimmune diseases. We have developed a strategy to produce an antiserum against autoantibodies for the treatment of T1DM. Non-obese diabetic (NOD) but not Balb/c mouse serum contains autoantibodies. Antisera were produced by immunizing Balb/c mice with affinity-purified IgG from NOD or BALB/c mice along with the immune adjuvant (hereafter, NIgG or BIgG, respectively). A bolus administration of NIgG significantly reduced serum autoantibodies, autoantibody-positive B lymphocytes in the spleens of NOD mice, mortality and morbidity of diabetes, blood glucose and islet immune infiltration, whereas it increased islet mass in NOD mice for at least 26 weeks. NIgG antiserum treatment has no significant effect on CD3(+), CD4(+) or CD8(+) T cells and B220(+) or CD19(+) B cells. BIgG also imparted a moderate therapeutic effect, although it was considerably lower than that of NIgG. NIgG did not cross-react with allogeneic serum. NIgG showed no effect on Balb/c mice....Continue Reading

References

Jan 1, 1992·The Journal of Clinical Investigation·D L KaufmanA J Tobin
May 22, 1986·The New England Journal of Medicine·G S Eisenbarth
Apr 3, 2002·Nature Medicine·Siri Atma W GreeleyHooman Noorchashm
Jul 18, 2002·Cell Research·Yuan Xin HuYong Lian Zhang
Nov 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·James W ThomasHolly G Mitchell
Jul 10, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoshihiro InoueToshiyuki Takai
Jan 8, 2008·Autoimmunity·Craig E Taplin, Jennifer M Barker
Aug 7, 2009·Autoimmunity·Yoav ArnsonHoward Amital
Sep 18, 2009·Annals of the New York Academy of Sciences·Joana Côrte-RealCarlos Penha-Gonçalves
Dec 29, 2009·Journal of Immunological Methods·Liping YuGeorge S Eisenbarth
Mar 20, 2010·Autoimmunity·Fariba Vaziri-SaniChristiane S Hampe
May 1, 2010·Nature·Jeffrey A BluestoneGeorge Eisenbarth
May 3, 2011·Trends in Immunology·Eliana MariñoShane T Grey
Sep 15, 2011·Expert Opinion on Biological Therapy·Shannon A Miller, Erin St Onge
May 16, 2012·Diabetes·Émmanuelle ÉnéePeter van Endert
Nov 7, 2012·Scandinavian Journal of Immunology·H SkärstrandF Vaziri-Sani

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