Abstract
Antibodies against soluble liver antigen/liver-pancreas (SLA/LP) have been associated with severe autoimmune hepatitis (AIH) and poor outcome, but most of these reports have focused on adult patients. The aim of this study was to assess the prevalence and clinical significance of anti-SLA/LP antibodies in a pediatric population with AIH. We developed a quantitative enzyme-linked immunoassay (ELISA), a Western blot (WB) and an immunoprecipitation assay (IPA) based on recombinant cDNA from activated Jurkat cells. The specificity of these tests was validated by testing 200 serum samples from healthy subjects, and from patients with liver and non-liver diseases. Anti-SLA/LP antibodies were found in patients with type 1 and type 2 AIH. The prevalence of these antibodies in patients with type 1 AIH was: 42% when tested by ELISA, 15% by WB and 50% by IPA. In patients with type 2 AIH, the prevalence rates were 42% by ELISA, 18% by WB and 44% by IPA. The mean titer values for anti-SLA/LP antibodies was significantly higher in type 2 AIH (1:1,300 +/- 339) than in type 1 AIH (1:600 +/- 71; p < 0.0001) and closely associated with higher titers of anti-liver kidney microsome type 1 (LKM1) and anti-liver cytosol type 1 (LC1) antibodies in se...Continue Reading
Citations
May 12, 2006·World Journal of Gastroenterology : WJG·Kazutaka KurokohchiShigeki Kuriyama
Jan 8, 2008·Autoimmunity·Francesca MedaCarlo Selmi
Jun 6, 2008·World Journal of Gastroenterology : WJG·Dimitrios-P BogdanosDiego Vergani
Mar 24, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Violaine EyraudCatherine Johanet
Jun 11, 2011·Digestive Diseases and Sciences·Chaojun HuYongzhe Li
Feb 13, 2013·Clinical & Developmental Immunology·Christiane RadzimskiLars Komorowski
Jan 9, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Cumali EfePaolo Muratori
Mar 23, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Antoni Xavier Torres-ColladoCarmen Gelpí
Mar 7, 2009·World Journal of Gastroenterology : WJG·Kathie BélandFernando Alvarez
May 14, 2010·Digestive Diseases and Sciences·Albert J Czaja
Nov 19, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Eve A Roberts
Jul 5, 2018·Drugs & Aging·Syed Rizvi, Samer Gawrieh
Dec 15, 2012·Expert Review of Gastroenterology & Hepatology·Maria I S AchenzaCarlo Selmi
Aug 15, 2020·Frontiers in Immunology·Naomi RichardsonDavid C Wraith
Oct 23, 2004·Autoimmunity·Susana VitozziFernando Alvarez
Sep 23, 2014·Journal of Clinical Laboratory Analysis·Danilo VillaltaUNKNOWN Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine, Italy
May 12, 2018·Archives of Oral Biology·Juliana Ximenes DamascenoCristiane Sá Roriz Fonteles