Anti-sphingosine-1-phosphate monoclonal antibodies inhibit angiogenesis and sub-retinal fibrosis in a murine model of laser-induced choroidal neovascularization.

Experimental Eye Research
Sergio CaballeroMaria Grant

Abstract

The efficacy of novel monoclonal antibodies that neutralize the pro-angiogenic mediator, sphingosine-1-phosphate (S1P), were tested using in vitro and in vivo angiogenesis models, including choroidal neovascularization (CNV) induced by laser disruption of Bruch's membrane. S1P receptor levels in human brain choroid plexus endothelial cells (CPEC), human lung microvascular endothelial cells, human retinal vascular endothelial cells, and circulating endothelial progenitor cells were examined by semi-quantitative PCR. The ability of murine or humanized anti-S1P monoclonal antibodies (mAbs) to inhibit S1P-mediated microvessel tube formation by CPEC on Matrigel was evaluated and capillary density in subcutaneous growth factor-loaded Matrigel plugs was determined following anti-S1P treatment. S1P promoted in vitro capillary tube formation in CPEC consistent with the presence of cognate S1P(1-5) receptor expression by these cells and the S1P antibody induced a dose-dependent reduction in microvessel tube formation. In a murine model of laser-induced rupture of Bruch's membrane, S1P was detected in posterior cups of mice receiving laser injury, but not in uninjured controls. Intravitreous injection of anti-S1P mAbs dramatically inhibit...Continue Reading

References

Feb 1, 1997·The British Journal of Ophthalmology·M KliffenP T de Jong
May 3, 2003·Nature Reviews. Molecular Cell Biology·Sarah Spiegel, Sheldon Milstien
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Junsuke IgarashiThomas Michel
Oct 18, 2003·Journal of Atherosclerosis and Thrombosis·Harunobu OzakiMenq-Jer Lee
Oct 28, 2003·Investigative Ophthalmology & Visual Science·Nilanjana SenguptaMaria B Grant
Oct 31, 2003·Journal of Cellular Biochemistry·Pascal N BernatchezMartin G Sirois
Mar 17, 2004·The Journal of Biological Chemistry·Brigitte Anliker, Jerold Chun
May 29, 2004·Prostaglandins & Other Lipid Mediators·Sung-Suk ChaeTimothy Hla
Jul 20, 2004·Journal of Cellular Biochemistry·Teresa Sanchez, Timothy Hla
Jul 20, 2004·Journal of Cellular Biochemistry·Julie Radeff-HuangJoan Heller Brown
Mar 4, 2005·Clinical and Experimental Pharmacology & Physiology·Tay Hwee KeeAlirio J Melendez
Mar 18, 2005·Annual Review of Immunology·Jason G Cyster
Jan 13, 2006·Trends in Molecular Medicine·Shannon E GardellJerold Chun
Oct 27, 2006·Expert Opinion on Emerging Drugs·Albert J Augustin, Indre Offermann
Oct 27, 2006·Investigative Ophthalmology & Visual Science·Lynn W MainesCharles D Smith
Jan 4, 2007·The American Journal of Pathology·Christina D KellerBurkhard Kleuser
Mar 28, 2007·American Journal of Ophthalmology·Marco A Zarbin
Mar 29, 2007·Journal of Thrombosis and Haemostasis : JTH·R GranataE Ghigo
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kyung-Hee ChangMaria B Grant
Aug 22, 2007·The Journal of Clinical Investigation·Athanasia SkouraTimothy Hla
Nov 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Kenneth R WattersonSarah Spiegel
Aug 9, 2008·Experimental Eye Research·James S SwaneyGlenn L Stoller

❮ Previous
Next ❯

Citations

Jun 16, 2012·Nature Reviews. Drug Discovery·Kang ZhangRobert N Weinreb
Aug 21, 2013·Nature Reviews. Drug Discovery·Gregory T KunkelSarah Spiegel
May 23, 2012·Pharmacological Reviews·Van A Doze, Dianne M Perez
Jan 19, 2010·American Journal of Respiratory Cell and Molecular Biology·Barry S SheaAndrew M Tager
Dec 17, 2009·Fibrogenesis & Tissue Repair·Clarice Zc Chen, Michael Raghunath
Jun 11, 2009·Journal of Lipid Research·Nicole O'BrienRoger Sabbadini
Jan 27, 2010·Journal of Lipid Research·Nora P RotsteinO Lorena German
May 7, 2013·Clinical Lipidology·Julia V BusikGavin E Reid
Sep 23, 2014·Expert Opinion on Investigational Drugs·Michael John TolentinoMichael Steven Tolentino
Jul 18, 2012·Biochimica Et Biophysica Acta·Nigel J PyneSusan Pyne
Mar 17, 2015·Experimental Eye Research·Keijiro IshikawaDavid R Hinton
Feb 12, 2011·British Journal of Pharmacology·Mirjam SchuchardtMarkus van der Giet
Dec 3, 2014·Advances in Biological Regulation·Long Shuang Huang, Viswanathan Natarajan
Jul 25, 2009·Immunobiology·Andreas WeigertBernhard Brüne
Jul 20, 2016·Expert Opinion on Drug Delivery·Victor M VillegasHarry W Flynn
Oct 3, 2018·British Journal of Pharmacology·Khaled ElmasryMohamed Al-Shabrawey
Feb 27, 2010·Journal of Cellular Biochemistry·Constantin BodeMarkus H Gräler
Jul 27, 2011·Critical Reviews in Biochemistry and Molecular Biology·Christopher R Gault, Lina M Obeid
Sep 5, 2013·The Journal of Biological Chemistry·Deron R HerrJerold Chun
Aug 2, 2017·Journal of Receptor and Signal Transduction Research·Mohd ArishAbdur Rub
Mar 7, 2020·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Xingrong LiuTimothy P Heffron
Mar 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nigel J Pyne, Susan Pyne
Jun 28, 2019·Frontiers in Cellular Neuroscience·M Victoria SimónNora P Rotstein
Jan 29, 2019·Frontiers in Pharmacology·Erjin WangMing Zeng
Nov 11, 2019·International Journal of Molecular Sciences·Chiara SassoliElisabetta Meacci
Dec 20, 2018·International Journal of Molecular Sciences·Hunter PorterNawajes Mandal
Dec 29, 2018·Metabolites·Connor N BrownUNKNOWN EYE-RISK consortium
Oct 19, 2019·Science·Andreane Cartier, Timothy Hla
Jan 10, 2021·Ophthalmology and Therapy·Tariq M AslamDavid C Hoyle
Nov 3, 2020·Drug Discovery Today·Matthew CollinsHanieh Khalili
Dec 1, 2020·Cellular Signalling·Chiara DonatiPaola Bruni
Sep 20, 2020·Journal of Lipid Research·M Victoria SimonNawajes Mandal
Feb 9, 2021·Journal of Lipid Research·Simon M VictoriaMandal Nawajes
May 9, 2021·The British Journal of Ophthalmology·Rasha A AlshaikhChristian Waeber
Oct 29, 2021·Indian Journal of Ophthalmology·Ezgi Naz Ensari DelioğluNurullah Çağıl

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