Anti-spike, Anti-nucleocapsid and Neutralizing Antibodies in SARS-CoV-2 Inpatients and Asymptomatic Individuals

Frontiers in Microbiology
Etienne BrochotFrancois Helle

Abstract

A better understanding of the anti-SARS-CoV-2 immune response is necessary to finely evaluate commercial serological assays but also to predict protection against reinfection and to help the development of vaccines. For this reason, we monitored the anti-SARS-CoV-2 antibody response in infected patients. In order to assess the time of seroconversion, we used 151 samples from 30 COVID-19 inpatients and monitored the detection kinetics of anti-S1, anti-S2, anti-RBD and anti-N antibodies with in-house ELISAs. We observed that specific antibodies were detectable in all inpatients 2 weeks post-symptom onset and that the detection of the SARS-CoV-2 Nucleocapsid and RBD was more sensitive than the detection of the S1 or S2 subunits. Using retroviral particles pseudotyped with the spike of the SARS-CoV-2, we also monitored the presence of neutralizing antibodies in these samples as well as 25 samples from asymptomatic individuals that were shown SARS-CoV-2 seropositive using commercial serological tests. Neutralizing antibodies reached a plateau 2 weeks post-symptom onset and then declined in the majority of inpatients but they were undetectable in 56% of asymptomatic patients. Our results indicate that the SARS-CoV-2 does not induce a...Continue Reading

References

Mar 29, 2014·Virus Research·Benjamin MeyerMarcel A Müller
Jan 25, 2020·The New England Journal of Medicine·Na ZhuUNKNOWN China Novel Coronavirus Investigating and Research Team
Mar 3, 2020·The Lancet Infectious Diseases·Long ChenYuan Shi
Mar 29, 2020·JAMA : the Journal of the American Medical Association·John D Roback, Jeannette Guarner
Apr 3, 2020·Nature·Roman WölfelClemens Wendtner
Apr 19, 2020·Nature Reviews. Immunology·Miyo Ota
May 12, 2020·JAMA : the Journal of the American Medical Association·Robert D KirkcaldyJohn T Brooks
May 18, 2020·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Alexander KrüttgenMichael Kleines
Jun 20, 2020·Nature·Davide F RobbianiMichel C Nussenzweig
Jul 4, 2020·Emerging Microbes & Infections·Li LiuZhiwei Chen
Jul 22, 2020·The Journal of Experimental Medicine·Fabian SchmidtPaul D Bieniasz
Aug 4, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kai WangAi-Long Huang
Aug 26, 2020·Nature Reviews. Immunology·Rebecca J Cox, Karl A Brokstad
Apr 18, 2020·MedRxiv : the Preprint Server for Health Sciences·Quanyi WangQuanyi Wang

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Citations

Feb 5, 2021·The Medical Journal of Australia·Heather F GiddingKristine Macartney
Feb 16, 2021·Frontiers in Immunology·Stephan SchlickeiserLeif G Hanitsch
Feb 21, 2021·Nature Communications·Adam K WheatleyStephen J Kent
Jan 4, 2021·Diagnostic Microbiology and Infectious Disease·Erika P OrnerChristopher W Farnsworth
Mar 20, 2021·Oxford Open Immunology·Diego CantoniNigel Temperton
May 1, 2021·Journal of Clinical Medicine·Aurélien AubryEtienne Brochot
May 3, 2021·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Wang-Da LiuShan-Chwen Chang
May 2, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Catharina GerhardsVerena Haselmann
May 27, 2021·Journal of Virology·Katherine LaTourretteHernan Garcia-Ruiz
Jun 3, 2021·Pathogens·Lorena JemeršićDragan Brnić
Jun 15, 2021·Vox Sanguinis·Marina IzakEilat Shinar
Jul 2, 2021·Journal of Virological Methods·Suraj JagtapRahul Roy
Aug 20, 2021·Laboratory Medicine·Elizabeth Smerczak
Sep 12, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Olivier BylickiJean-Nicolas Tournier
Oct 2, 2021·Geriatrics & Gerontology International·Julien MoyetFrédéric Bloch
Sep 21, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Gururaj Rao DeshpandeGajanan Sapkal
Nov 12, 2021·Australian and New Zealand Journal of Public Health·Tasnim HasanMatthew V N O'Sullivan
Nov 25, 2021·The Journal of General Virology·Aleksander SzymczakKrystyna Dąbrowska

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Datasets Mentioned

BETA
MN908947

Methods Mentioned

BETA
ELISA
ELISAs

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