Anti-thrombosis Effects and Mechanisms by Xueshuantong Capsule Under Different Flow Conditions

Frontiers in Pharmacology
Shu-Xian HanFu-Long Liao

Abstract

Xueshuantong capsule (XST) is a patented traditional Chinese medicine used for the prevention and treatment of thrombosis. The molecular mechanism of anti-thrombotic effect of XST was investigated through the cross-talk among the platelets/leukocytes, endothelial cells (ECs), and flow shear stress. The Bioflux 1000 system was used to generate two levels of shear stress conditions: 0.1 and 0.9 Pa. Bioflux Metamorph microscopic imaging system was used to analyze the adhesion cell numbers. Protein expressions were detected by western blotting and flow cytometry. The flow-cytometry results showed that under 0.1 Pa flow, XST decreased ADP induced platelets CD62p surface expression in a concentration-dependent manner. Under 0.9 Pa flow, XST at a concentration of 0.15 g⋅L-1 reduced the platelets activation by 29.5%, and aspirin (ASA) showed no inhibitory effects. XST showed similar efficiency on monocytes adhesion both under 0.1 and 0.9 Pa flow conditions, and the inhibition rate was 30.2 and 28.3%, respectively. Under 0.9 Pa flow, the anti-adhesive effects of XST might be associated with the suppression of VE-cadherin and Cx43 in HUVECs. Blood flow not only acts as a drug transporter, but also exerts its effects to influence the phar...Continue Reading

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Citations

Jan 24, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dandan WangYaozu Xiang
Sep 3, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Paula Mendonça LeiteRachel Oliveira Castilho
Sep 22, 2020·Complementary Therapies in Medicine·Jie-Ping LiQin Li

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Methods Mentioned

BETA
Assay
electrophoresis
flow cytometry

Software Mentioned

Bioflux Montage
Statistical Package for the Social Sciences ( SPSS

Related Concepts

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