Anti-TLR2 antibody triggers oxidative phosphorylation in microglia and increases phagocytosis of β-amyloid

Journal of Neuroinflammation
A Rubio-AraizMarina A Lynch

Abstract

Microglia are multifunctional cells that are primarily neuroprotective and a deficit in their functional integrity is likely to be a contributory factor in the deteriorating neuronal function that occurs with age and neurodegeneration. One aspect of microglial dysfunction is reduced phagocytosis, and this is believed to contribute to the accumulation of amyloid-β (Aβ) in Alzheimer's disease (AD). Therefore, improving phagocytosis should be beneficial in limiting the amyloidosis that characterises AD. Here, we investigated whether an antibody that targets toll-like receptor (TLR)2 might attenuate the inflammatory and metabolic changes induced by lipopolysaccharide (LPS) and amyloid-β. The impact on phagocytosis was assessed by immunohistochemistry. We evaluated the metabolic changes with the SeaHorse Extracellular Flux Analyser and studied the expression of key enzymes driving glycolysis by western blotting. For all experiments, statistical significance was determined by unpaired Student's t test and two-way analysis of variance (ANOVA). We have reported that, when exposed to an inflammatory stimulus, microglia switch their metabolism towards the metabolically- inefficient glycolysis; this potentially impacts on metabolically de...Continue Reading

References

Sep 13, 2001·The European Journal of Neuroscience·X QiaoS M Paul
Feb 18, 2004·Current Protein & Peptide Science·Yann Verdier, Botond Penke
Nov 5, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jessica Koenigsknecht, Gary Landreth
Sep 9, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jessica Koenigsknecht-Talboo, Gary E Landreth
Sep 21, 2006·Brain : a Journal of Neurology·Kazuki TaharaKen-ichiro Fukuchi
May 30, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Karine L RichardSerge Rivest
Jul 8, 2008·Nature Immunology·Annett HalleDouglas T Golenbock
Sep 25, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Erin G Reed-GeaghanGary E Landreth
May 26, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Juan-Carlos Rodríguez-PradosLisardo Boscá
Sep 22, 2010·Nature Immunology·Subhra K Biswas, Alberto Mantovani
Oct 15, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patrick VollmarBernhard Hemmer
Dec 27, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shirong LiuKlaus Fassbender
Jun 16, 2012·Journal of Neuroinflammation·Aedín M MinogueMarina A Lynch
Jul 18, 2012·Nature·Ben LuKevin J Tracey
Feb 11, 2014·Journal of Neuroscience Research·J Gimeno-BayónN Mahy
Feb 22, 2014·Current Opinion in Immunology·Julien Moretti, J Magarian Blander
Aug 5, 2014·Nature Immunology·Stanley Ching-Cheng HuangEdward J Pearce
Apr 18, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Ahmed TawakolLisardo Boscá
Jun 6, 2015·Cell Research·Beth Kelly, Luke A J O'Neill
Jun 30, 2015·Cell Reports·Jong-Seok MoonAugustine M K Choi
Nov 6, 2015·Journal of Neuroinflammation·Jonathan D CherryM Kerry O'Banion
Sep 28, 2016·Trends in Biochemical Sciences·Yuan HeGabriel Núñez
Oct 26, 2016·Nature Communications·Min XieDaolin Tang
Nov 11, 2016·Molecular Neurobiology·Anthony LyonsMarina A Lynch
Apr 12, 2017·Trends in Immunology·Jan Van den BosscheDeepthi Menon
Apr 26, 2017·The EMBO Journal·Simonetta Camandola, Mark P Mattson
Dec 17, 2017·Immunological Reviews·Mark M Hughes, Luke A J O'Neill

❮ Previous
Next ❯

Citations

Nov 7, 2019·Journal of Neurochemistry·Israel C NnahMarianne Wessling-Resnick
Sep 12, 2019·Frontiers in Pharmacology·Knut BiberThomas Möller
Mar 3, 2020·Frontiers in Cellular Neuroscience·Ruqayya AfridiKyoungho Suk
Sep 16, 2020·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Patrick T Ronaldson, Thomas P Davis
Apr 9, 2020·Frontiers in Immunology·Clotilde Lauro, Cristina Limatola
Nov 17, 2020·Frontiers in Cellular Neuroscience·Daniel C Shippy, Tyler K Ulland
Nov 20, 2020·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Wenjun DengChanghong Xing
Mar 16, 2021·Frontiers in Immunology·Lauren H FairleyAnna M Barron
Sep 23, 2020·Trends in Neurosciences·Louis-Philippe BernierBrian A MacVicar
Jun 12, 2021·Communications Biology·Marie-Victoire Guillot-SestierMarina A Lynch
Jul 28, 2021·Journal of Neuroimmunology·Marlene Thorsen MorchTrevor Owens
Aug 16, 2021·Ageing Research Reviews·Zoë P Van AckerWim Annaert

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
Assay

Software Mentioned

SeaHorse XF24
ImageJ
LAS AF Lite

Related Concepts

Related Feeds

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Alzheimer's Disease: Microglia

Microglia are a type of glial cell found throughout the brain and spinal cord. Microglia have been found to be associated with Alzheimer's disease development and progression. Here are the latest discoveries pertaining to Alzheimer's disease and microglia.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.