PMID: 9641513Jun 26, 1998Paper

Anti-transfer RNA antibodies in two patients with pulmonary fibrosis, Raynaud's phenomenon and polyarthritis

Clinical Rheumatology
Y OhosoneT Mimori

Abstract

Patient W.S. (a 61-year-old woman) and patient T.M. (a 41-year-old man) developed recurrent fevers, polyarthritis, Raynaud's phenomenon and interstitial pulmonary fibrosis without apparent polymyositis. From HeLa cell extracts, sera from both patients immunoprecipitated all species of intact and deproteinised tRNAs. To identify the antibody binding site more precisely, tRNAs transcribed in vitro from cloned Escherichia coli tRNA genes and various mutants were prepared and used as antigens for immunoprecipitation. When the TpsiC loop, or the D loop were deleted, such mutants were not bound by both sera, suggesting that the D and TpsiC loops were required for antibody binding. Abrogation of tRNA binding occurred when 18G of tRNATrp was replaced with 18A to break the tertiary L-shape structure of tRNA. These results strongly suggest that sera from W.S. and T.M. recognise the tertiary conformation of L-shaped tRNA which is constructed with both D and TpsiC loops. These autoantibodies may also serve as a marker for a new subset of patients with connective tissue diseases that is distinct from anti-aminoacyl-tRNA synthetase syndrome.

References

Mar 1, 1990·The American Journal of Medicine·I N Targoff, F C Arnett
Aug 29, 1986·Biochemical and Biophysical Research Communications·J Sri-WidadaP Jeanteur
May 1, 1986·The Journal of Experimental Medicine·C C BunnM B Mathews
Jul 21, 1984·British Medical Journal·R M BernsteinG R Hughes

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Citations

Sep 29, 1998·Arthritis and Rheumatism·Y OhosoneT Mimori
Dec 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jing-Dong YeJoseph A Piccirilli
Sep 21, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Joseph A Piccirilli, Yelena Koldobskaya
Jul 12, 2014·Expert Review of Molecular Diagnostics·Zonglin HuChristopher A Elkins
Sep 7, 2016·Journal of Molecular Biology·Yaming ShaoJoseph A Piccirilli

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