Anti-tumor effect of cisplatin in human oral squamous cell carcinoma was enhanced by andrographolide via upregulation of phospho-p53 in vitro and in vivo

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Songjie ChenHui Zhao

Abstract

Oral squamous cell carcinoma is one of the most common neoplasm in the world. Despite the improvements in diagnosis and treatment, the outcome is still poor now. Thus, the development of novel therapeuticapproaches is needed. The aim of this study is to assess the synergistic anti-tumor effect of andrographolide with cisplatin (DDP) in oral squamous cell carcinoma CAL-27 cells in vitro and in vivo. We performed Cell Counting Kit-8 proliferation assay, apoptosis assay, and western blotting on CAL-27 cells treated with andrographolide, DDP or the combination in vitro. In vivo, we also treated CAL-27 xenografts with andrographolide or the combination, and performed terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling assay and immunohistochemical analysis of Ki-67. The results showed the combination of andrographolide and DDP synergistically inhibited CAL-27 cell proliferation in vitro and caused tumor regression in vivo in the CAL-27 xenografts. In addition, the synergistic anti-tumor effect of andrographolide with synergistic was due to an enhanced apoptosis. Moreover, the combination therapy upregulated the expression level of p-p53 in vitro and decreased Ki-67 expression in vivo. Our data ...Continue Reading

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Citations

Mar 29, 2019·Journal of Biochemistry·Atsushi YasukochiSeiji Nakamura
Oct 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rasha H Al-SerwiNehal Elsherbiny
Apr 30, 2020·Mini Reviews in Medicinal Chemistry·Meng HaoHui Xu

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Methods Mentioned

BETA
xenograft
xenografts
flow
FCS
protein assay
electrophoresis
flow cytometry

Software Mentioned

FCS Express
GLOBOCAN

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