Anti-tumor effect of PEG-coated PLGA nanoparticles of febuxostat on A549 non-small cell lung cancer cells.

3 Biotech
Mohammad Y AlfaifiShadab Md

Abstract

In the present study, febuxostat (FBX)-loaded PEG-coated PLGA nanoparticles (FBX-PLGA-PEG) were developed and its anticancer activity on lung cancer cells was evaluated. FBX-PLGA-PEG were prepared by nanoprecipitation technique and characterized for particle size, size distribution, entrapment efficiency, and in vitro drug release study. The optimized formulations were used to evaluate cell viability, apoptosis, cell cycle, and caspase activity in A549 lung cancer cells. The optimized formulation showed spherical particle with average particle size of 180 ± 4.72 nm, particle-size distribution 0.223 ± 0.003, entrapment efficiency (70 ± 2.56%), and drug release (99.1 ± 2.33%) at 12 h. MTT cytotoxicity assay showed better cytotoxic potential of FBX-NPs than FBX solution against NSCLC A549 cells. The lower IC50 of FBX-NP (52.62 ± 2.52 µg/mL) compared to FBX (68.0 ± 4.12 µg/mL) are suggestive of a potential cytotoxic effect of nano-formulation compared to the drug itself. Furthermore, flow cytometry analysis showed significantly higher percentage of total apoptotic cells in FBX-NPs (10.38 ± 1.57%) as compared to FBX solution (2.76 ± 0.17%) showed strong proapoptotic potential of FBX nano-formulation. The increased caspase activity a...Continue Reading

References

Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·Q P DouP L Will
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Sep 5, 2002·Nature Reviews. Cancer·Suzanne Cory, Jerry M Adams
Mar 11, 2010·Handbook of Experimental Pharmacology·Vladimir P Torchilin
Aug 4, 2010·Journal of the National Cancer Institute·Adi F GazdarJohn D Minna
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Feb 23, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fabienne DanhierVéronique Préat
May 12, 2012·Polymers·Hirenkumar K Makadia, Steven J Siegel
Nov 1, 2012·Indian Journal of Pharmaceutical Sciences·Manisha Bisht, S S Bist
Sep 12, 2015·Oncotarget·Wenjuan XuXuegang Sun
Oct 13, 2015·Advanced Drug Delivery Reviews·Jung Soo SukLaura M Ensign
Dec 3, 2015·Cold Spring Harbor Perspectives in Biology·Douglas R Green, Fabien Llambi
Dec 27, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Farha Masood
Mar 8, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kuan Yin HsiaoShyh Ming Kuo
Jul 15, 2016·Current Drug Delivery·Suryakanta SwainSitty Manohar Babu
Jul 15, 2016·Translational Lung Cancer Research·Cecilia Zappa, Shaker A Mousa
Dec 28, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Qing-Sheng ZhaoJing Liu
Jun 7, 2017·BMC Complementary and Alternative Medicine·Kitti PumiputavonPathrapol Lithanatudom
Aug 5, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohd Abul Kalam, Aws Alshamsan
Sep 26, 2017·International Journal of Pharmaceutics·Hassan A AlmoustafaZamri Chik
May 5, 2018·International Journal of Biological Sciences·Wenfeng LinPeng Huang
Jun 22, 2018·The Journal of Physical Chemistry. B·Natalia WilkoszMariusz Kepczynski
Sep 12, 2018·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Anmar M NassirMohammad R Ain
Oct 13, 2018·Nature Reviews. Drug Discovery·Sudeep PushpakomMunir Pirmohamed
Nov 16, 2018·Frontiers in Pharmacology·Clara FernandesMayur C Yergeri
Jan 27, 2019·Therapeutic Delivery·Gizem BorAnan Yaghmur
Jun 16, 2019·Journal of Functional Biomaterials·Marcus HillMarie E Migaud

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.