Anti-tumor effect of the alphavirus-based virus-like particle vector expressing prostate-specific antigen in a HLA-DR transgenic mouse model of prostate cancer

Vaccine
V RiabovE N Klyushnenkova

Abstract

The goal of this study was to determine if an alphavirus-based vaccine encoding human Prostate-Specific Antigen (PSA) could generate an effective anti-tumor immune response in a stringent mouse model of prostate cancer. DR2bxPSA F1 male mice expressing human PSA and HLA-DRB1(*)1501 transgenes were vaccinated with virus-like particle vector encoding PSA (VLPV-PSA) followed by the challenge with Transgenic Adenocarcinoma of Mouse Prostate cells engineered to express PSA (TRAMP-PSA). PSA-specific cellular and humoral immune responses were measured before and after tumor challenge. PSA and CD8 reactivity in the tumors was detected by immunohistochemistry. Tumor growth was compared in vaccinated and control groups. We found that VLPV-PSA could infect mouse dendritic cells in vitro and induce a robust PSA-specific immune response in vivo. A substantial proportion of splenic CD8 T cells (19.6 ± 7.4%) produced IFNγ in response to the immunodominant peptide PSA(65-73). In the blood of vaccinated mice, 18.4 ± 4.1% of CD8 T cells were PSA-specific as determined by the staining with H-2D(b)/PSA(65-73) dextramers. VLPV-PSA vaccination also strongly stimulated production of IgG2a/b anti-PSA antibodies. Tumors in vaccinated mice showed low le...Continue Reading

References

May 11, 1999·JAMA : the Journal of the American Medical Association·C R PoundP C Walsh
Jan 7, 2000·Journal of Virology·G H MacDonald, R E Johnston
Apr 20, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Elena N KlyushnenkovaRichard B Alexander
Nov 22, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Stacie M GoldbergJedd D Wolchok
Jul 4, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Robert J DursoWilliam C Olson
Aug 19, 2007·Vaccine·Richard J Anderson, Joerg Schneider
Feb 15, 2008·Cancer Immunology, Immunotherapy : CII·Vafa ShahabiPaulo Maciag
Mar 14, 2008·Nature Reviews. Cancer·Hans LiljaAndrew J Vickers
Aug 10, 2010·Virus Research·Jose I QuetglasCristian Smerdou
Jun 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·James L Gulley, Charles G Drake
Aug 10, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Edward Cha, Lawrence Fong
May 30, 2012·Nature Reviews. Urology·Dev KaranJ Brantley Thrasher
Jan 31, 2013·BMJ : British Medical Journal·Timothy J Wilt, Hashim U Ahmed
Apr 30, 2014·Cancer Immunology Research·James L GulleyJeffrey Schlom
May 20, 2014·Immunologic Research·Matthew A UhlmanDavid M Lubaroff
Aug 7, 2014·Oncoimmunology·Ravi A MadanJames L Gulley

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Citations

Apr 8, 2016·Expert Review of Vaccines·Peter PushkoIrina Tretyakova
Nov 10, 2016·Vaccines·Kenneth Lundstrom
Sep 4, 2018·Expert Review of Vaccines·Braeden DonaldsonVernon K Ward
Feb 27, 2019·Nanomedicine·Ru WenAmmad Ahmad Farooqi
Jul 24, 2020·International Journal of Molecular Sciences·Kenneth Lundstrom
Oct 27, 2020·Frontiers in Immunology·Morgan BrisseHinh Ly

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