Anti-Tumor Effects of Bak-Proteoliposomes against Glioblastoma

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Lavinia LiguoriJean-Luc Lenormand

Abstract

Despite palliative treatments, glioblastoma (GBM) remains a devastating malignancy with a mean survival of about 15 months after diagnosis. Programmed cell-death is de-regulated in almost all GBM and the re-activation of the mitochondrial apoptotic pathway through exogenous bioactive proteins may represent a powerful therapeutic tool to treat multidrug resistant GBM. We have reported that human Bak protein integrated in Liposomes (LB) was able, in vitro, to activate the mitochondrial apoptotic pathway in colon cancer cells. To evaluate the anti-tumor effects of LB on GBM, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays and Western blot analysis were performed on GL26 murine cell line. LB treatment shows a dose-dependent inhibition of cell viability, followed by an up-regulation of Bax and a down-modulation of JNK1 proteins. In GL26-bearing mice, two different routes of administration were tested: intra-tumor and intravenous. Biodistribution, tumor growth and animal survival rates were followed. LB show long-lasting tumor accumulation. Moreover, the intra-tumor administration of LB induces tumor growth delay and total tumor regression in about 40% of treated mice, while the intravenous injection leads t...Continue Reading

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Citations

Apr 6, 2016·Cancer Letters·Tao WangWei Duan
May 12, 2021·Life Science Alliance·Géraldine MayeuxJean-Luc Lenormand

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Methods Mentioned

BETA
electrophoresis
Assay

Software Mentioned

Prism
gel analyzer
InvivoScope

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