Anti-tumor Efficacy Study using Irreversible Electroporation and Doxorubicin-loaded Polymeric Micelles

ACS Macro Letters
Jun ZhaoMarites P Melancon

Abstract

Irreversible electroporation (IRE) is a novel non-thermal ablative treatment for cancer patients with unresectable tumor. IRE kills tumor cells by applying a strong electric field across the cell membrane, thereby creating irreparable pores. Compared to conventional thermal ablation, IRE is effective in perivascular tissues and can preserve the surrounding sensitive structures. However, tumor cells may survive in the regions exposed to insufficient electric field strength, and cause tumor relapse afterwards. We prepared a doxorubicin-loaded polymeric micelles system (M-Dox) using oil-in-water emulsion. The resultant M-Dox was 37.9 ± 3.2 nm in size with a Dox loading of 4.3% by weight. M-Dox is toxic to multiple human cancer cell lines with IC50 values in nanomolar and micromolar range. When combined with IRE in a hepatic carcinoma mouse xenograft model, the tumor treated with the combination therapy (IRE + M-Dox) was the highest in both M-Dox uptake and percentage of necrosis. Immunohistochemical staining also confirmed that the fewest proliferating cells were present after the combination therapy. Our data suggested that M-Dox was an effective adjuvant treatment to enhance the anti-tumor efficacy of IRE.

References

Feb 11, 1997·Biochimica Et Biophysica Acta·M J JaroszeskiR Heller
Mar 10, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M J JaroszeskiR Heller
Apr 10, 2004·Advances in Colloid and Interface Science·Tharwat TadrosC Solans
Mar 18, 2005·Annals of Biomedical Engineering·R V DavalosB Rubinsky
Sep 15, 2005·Journal of the American Chemical Society·Jianzhong Du, Steven P Armes
Jan 24, 2007·Technology in Cancer Research & Treatment·Boris RubinskyPaul Mikus
Sep 17, 2009·Annals of Biomedical Engineering·Robert E Neal, Rafael V Davalos
Nov 4, 2009·International Journal of Heat and Mass Transfer·Yair Granot, Boris Rubinsky
Dec 21, 2010·Journal of Biomedical Materials Research. Part a·Wen ZhuFu Xi
Dec 30, 2010·IEEE Transactions on Bio-medical Engineering·Christopher B ArenaRafael V Davalos
Feb 12, 2011·HPB : the Official Journal of the International Hepato Pancreato Biliary Association·Kevin P CharpentierDamian E Dupuy
Jan 19, 2012·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·T R GowrishankarJ C Weaver
Oct 24, 2012·Journal of Surgical Oncology·Robert CannonRobert C G Martin
Aug 27, 2014·International Journal of Cancer. Journal International Du Cancer·Lu WangCong Li
Nov 12, 2014·Nature Reviews. Clinical Oncology·Lynda WyldGraeme J Poston

❮ Previous
Next ❯

Citations

Oct 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li TianMarites P Melancon
Apr 23, 2021·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Raniv D RojoMarites P Melancon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.