Anti-Vasculogenic Activity of a Polysaccharide Derived from Brittle Star via Inhibition of VEGF, Paxillin and MMP-9

Iranian Journal of Biotechnology
Javad BahararaMarziyeh Musavi

Abstract

Background: Bioactive compounds such as terpenoids, chondroitin sulfate, and polysaccharides with added value can be found in prestine marine creatures. These compounds often do have highly valuable therapeutic applications such as being antioxidant, antitumorogenic, anti-inflammatory and anti-angiogenic. For the latter, varieties of angiogenesis factors can suppress this issue within the bodily tissues. Objectives: The anti-angiogenic and anti-metastatic capacity of a polysaccharide derived from brittle star was investigated. Material and Methods: The anti-proliferative effect of derived polysaccharide on umbilical vein endothelial cells (HUVEC) was measured using MTT (dimethyl thiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. The anti-angiogenic effect of the isolated polysaccharide was examined by Chorioallantoic membrane (CAM) assay. The transcriptional expression of VEGF (Vascular Endothelial Growth Factor) was evaluated by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). The anti-metastatic activity was investigated via scratch-wound healing assay. The levels of Paxillin and Matrix Metalloproteinase-9 (MMP-9) expression were analyzed by RT-PCR. Statistical analysis and mean comparisons (p< 0.05) were carried...Continue Reading

References

Dec 16, 2005·Nature·Napoleone Ferrara, Robert S Kerbel
May 16, 2006·Trends in Pharmacological Sciences·Tai-Ping FanRicky N S Wong
Dec 15, 2006·The New England Journal of Medicine·Alan SandlerDavid H Johnson
Jan 12, 2007·The New England Journal of Medicine·Robert J MotzerRobert A Figlin
Mar 31, 2007·Nature Reviews. Drug Discovery·Judah Folkman
Dec 28, 2007·The New England Journal of Medicine·Kathy MillerNancy E Davidson
Jan 15, 2008·Drug Discovery Today·Janice M Reichert, Julia B Wenger
Feb 27, 2008·Biotechnology Advances·Narsinh L ThakurWerner E G Müller
Aug 12, 2008·Biochimica Et Biophysica Acta·Megumi IiizumiKounosuke Watabe
Dec 15, 2010·Molecular Biology of the Cell·Nicholas O Deakin, Christopher E Turner
Sep 29, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christopher LieuScott Kopetz
Apr 19, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Fang LiuXiangyang Zou
Jun 15, 2012·Thrombosis Research·Francesca Elice, Francesco Rodeghiero
Jul 26, 2012·International Journal of Biological Macromolecules·Tao XinGuangjie Sui
Sep 5, 2012·Carbohydrate Polymers·Aizhen ZongFengshan Wang
Dec 4, 2012·Biochemical and Biophysical Research Communications·Xiangyi LuCheng Luo
Dec 25, 2012·Natural Product Reports·John W BluntMichèle R Prinsep
Feb 26, 2013·Journal of Reproductive Immunology·Andrew K EdwardsChandrakant Tayade
Mar 20, 2013·Microvascular Research·Celina Maria P Guerra DoreEdda Lisboa Leite
Oct 24, 2013·Environmental Toxicology and Pharmacology·Kalimuthu SenthilkumarSe-Kwon Kim
Aug 19, 2014·Carbohydrate Polymers·Pang MyronSujjat Al Azad
May 1, 2012·Food Chemistry·Zhi-Heng HePaul Pui-Hay But

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May 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Claudio LuparelloMirella Vazzana

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