PMID: 6126538Jul 1, 1982Paper

Anti-warfarin antibody preparation and its characterization for radioimmunoassay

The Journal of Pharmacy and Pharmacology
H SatohS Satoh

Abstract

Anti-warfarin antiserum was prepared in rabbits by immunization with a synthesized warfarin antigen, 4'-azo-warfarin human serum albumin, which possesses two enantiomorphic haptenic sites of warfarin on the molecule. The antiserum recognized both R- and S-warfarin to the same degree, 50% cross reactivities of racemic warfarin, respectively. One of the warfarin metabolites, racemic warfarin alcohol, showed 1% cross reactivity, and R- or S-warfarin alcohol have half the reactivity of the racemic alcohol. Rabbit plasma warfarin levels were determined by radioimmunoassay using this antiserum and racemic [14C]warfarin and by fluorometric assay after isolation by thin layer chromatography. After a single administration of warfarin (2 mg kg-1 orally or 500 microgram kg-1 i.v.), the plasma levels determined by both assay methods showed a good correlation (r = 0.97, P less than 0.001, Y = 1.04-0.09). The results show that the radioimmunoassay can determine total plasma warfarin without interference of plasma metabolite. The applicability and limitation of the radioimmunoassay for pharmacokinetic study are discussed.

References

Nov 1, 1975·The Journal of Pharmacy and Pharmacology·K Bachmann, A M Burkman
Oct 1, 1978·Journal of Medicinal Chemistry·M J FascoL S Kaminsky
Oct 1, 1976·Biochemical Pharmacology·L R PohlJ W Fenton
Aug 1, 1974·Clinical Pharmacology and Therapeutics·R A O'Reilly
Jun 1, 1974·The Journal of Clinical Investigation·R J LewisW Schary
Nov 1, 1970·Journal of Medicinal Chemistry·W F TragerW A Garland
Dec 1, 1970·Biochemical Medicine·R J LewisM Carlstrom
Mar 1, 1981·Journal of Pharmacobio-dynamics·M Maeda, A Tsuji

Citations

Nov 16, 2001·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·M J Ruedas RamaA Molina Díaz

Related Concepts

Cross Reactions
Radioimmunoassay
Molecular Stereochemistry
Gen-Warfarin

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