Abstract
The present paper was designed to investigate the effect of pine pollen against aging in human diploid fibroblast 2BS cells and in an accelerated aging model, which was established by subcutaneous injections with D-galactose daily for 8 weeks in C57BL/6J mice. Pine pollen (1 mg/mL and 2 mg/mL) is proved to delay the replicative senescence of 2BS cells as evidenced by enhanced cell proliferation, decreased SA-β-Gal activity, and reversed expression of senescence-associated molecular markers, such as p53, p21(Waf1), p16(INK4a), PTEN, and p27(Kip1) in late PD cells. Besides, pine pollen reversed D-galactose-induced aging effects in neural activity and inflammatory cytokine levels, as indicated by improved memory latency time and reduced error rate in step-down test and decreased concentrations of IL-6 and TNF-α in model mice. Similar to the role of AGEs (advanced glycation endproducts) formation inhibitor aminoguanidine (AG), pine pollen inhibited D-galactose-induced increment of AGEs levels thus reversed the aging phenotypes in model mice. Furthermore, the declined antioxidant activity was obviously reversed upon pine pollen treatment, which may account for its inhibitory effect on nonenzymatic glycation (NEG) in vivo. Our findin...Continue Reading
References
Aug 28, 1992·Science·E R Stadtman
Apr 21, 1995·Mechanisms of Ageing and Development·J LiT Tong
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·G P DimriO Pereira-Smith
Apr 1, 1995·Trends in Neurosciences·M A SmithG Perry
Jan 1, 1994·Mechanisms of Ageing and Development·Z TangT Tong
Jul 16, 1999·Mechanisms of Ageing and Development·X SongY M Li
Jun 1, 2000·Experimental Gerontology·F Bringold, M Serrano
May 8, 2001·Molecular and Cellular Biology·K Alexander, P W Hinds
Mar 26, 2003·Biogerontology·Shih-Ching HoReY-Yih Wu
Mar 1, 1965·Experimental Cell Research·L HAYFLICK
Dec 9, 2003·Mechanisms of Ageing and Development·Peichang WangTanjun Tong
Jan 11, 2005·Behavioural Brain Research·Haifeng WeiWei Li
Apr 7, 2005·FEBS Letters·Massimo De MartinisLia Ginaldi
Aug 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jane TianJames T Handa
May 20, 2006·Journal of Neuroscience Research·Xu CuiJiankang Liu
Jun 24, 2006·Drug Discovery Today·V Prakash Reddy, Ayse Beyaz
Dec 19, 2006·Life Sciences·Shi-Yan LiJun Ren
Mar 16, 2007·European Journal of Nutrition·Paul UriosMichel Sternberg
Jul 21, 2007·Journal of Translational Medicine·David P MangiameliSteven K Libutti
Sep 11, 2007·Atherosclerosis·Giuseppina Basta
Mar 11, 2008·Biochemical and Biophysical Research Communications·Ming LeiGang Hu
Dec 25, 2008·Phytotherapy Research : PTR·Kyung-Hee LeeEun-Mi Choi
Jan 17, 2009·Phytotherapy Research : PTR·Kyung-Hee Lee, Eun-Mi Choi
Dec 9, 2009·Free Radical Biology & Medicine·Srinath PashikantiDaniel Cervantes-Laurean
May 19, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Richard D SembaLuigi Ferrucci
Jun 3, 2010·Biomedical and Environmental Sciences : BES·Gen-Xiang MaoZhen Wang
Nov 3, 2010·Mechanisms of Ageing and Development·Gen-xiang MaoZhen Wang
Jun 2, 2011·International Journal of Alzheimer's Disease·Anil KumarSamrita Dogra
Jul 2, 2011·Journal of Pineal Research·Dae Young YooIn Koo Hwang
Citations
Nov 8, 2015·Journal of Cardiovascular Electrophysiology·Mingxian ChenShenghua Zhou
Apr 23, 2013·Biotechnology Progress·Ding LiXiaoning Wang
Jan 1, 2014·BioMed Research International·Claudia MeindlEleonore Fröhlich
Apr 11, 2017·Journal of Pharmacopuncture·Mahdieh Jafari ShahroudiHossein Hosseinzadeh
Jul 3, 2018·Journal of the Science of Food and Agriculture·Yue ShanJinwei Li
Nov 14, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chun DangJunwei Hao
Jan 13, 2018·International Journal of Molecular Medicine·Liandong ZhangTie Chong
Mar 31, 2018·Oxidative Medicine and Cellular Longevity·Lei ZhangLin Wang
Feb 14, 2017·Oxidative Medicine and Cellular Longevity·Peina WangYan-Zhong Chang
Oct 30, 2018·Frontiers in Microbiology·Xinpeng JiangJunwei Ge
Nov 12, 2019·Frontiers in Pharmacology·Jing-Yu QianZheng-Ping Yu
Jul 14, 2020·Oxidative Medicine and Cellular Longevity·Bauyrzhan UmbayevDenis Bulanin
Dec 31, 2016·Metabolic Brain Disease·Feng YuanTieli Dong
Dec 13, 2018·Oxidative Medicine and Cellular Longevity·Hao HuangLiegang Liu
Oct 16, 2019·Oxidative Medicine and Cellular Longevity·Gen-Xiang MaoJing Yan
Mar 14, 2021·Biological Chemistry·Chao DuHe Qian
Apr 20, 2021·Dose-response : a Publication of International Hormesis Society·Yu-Zhong ChenJia-Ying Xu
Jun 9, 2021·Metallomics : Integrated Biometal Science·Yabo LiuZhiyong Zhang