PMID: 7541309Apr 1, 1995Paper

Antiallergic activity of loratadine: inhibition of leukotriene C4 release from human leucocytes

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
A MiadonnaA Tedeschi

Abstract

The H1 antagonist loratadine has the capacity to inhibit histamine release from human basophils. The aim of this study was to investigate whether loratadine can also inhibit leukotriene C4 (LTC4) release from human leucocytes. Basophil-enriched mononuclear cell suspensions were prepared by centrifugation of peripheral venous blood (n = 10) on discontinuous Percoll gradients. Leucocytes were stimulated with anti-IgE, N-formylmethionyl-leucyl-phenylalanine (FMLP) and Ca2+ ionophore A23187; immunoreactive (i) LTC4 release in the cell supernatant was measured by a competitive radioimmunoassay and histamine release was evaluated by an automated fluorometric technique. Loratadine, in the concentration range of 1-50 microM, exerted a dose-dependent inhibitory effect on IgE-mediated and IgE-independent histamine and iLTC4 release. The concentrations inhibiting 50% of histamine release were 30 microM (anti-IgE), 27 microM (FMLP) and 19 microM (Ca2+ ionophore A23187). The concentrations inhibiting 50% of iLTC4 release were 2.3 microM (anti-IgE). 11 microM (FMLP) and 1.7 microM (Ca2+ ionophore A23187). The inhibitory activity on iLTC4 release was optimal after preincubation for 2 h at 37 degrees C, and was no longer evident when leucocyte...Continue Reading

References

Oct 1, 1985·The Journal of Allergy and Clinical Immunology·E J Leonard, A Skeel
Nov 1, 1988·Journal of the American Academy of Dermatology·E W Monroe
Apr 15, 1967·Nature·M Peterlik, W Waldhäusl
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L LevineE J Corey
Sep 1, 1994·International Archives of Allergy and Immunology·A MiadonnaA Tedeschi

❮ Previous
Next ❯

Citations

Jan 28, 1998·Clinical Therapeutics·J L MenardoW Czarlewski
May 5, 2000·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·J W GeorgitisR Berkowitz
Dec 31, 1997·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G CiprandiG W Canonica
Mar 9, 1999·The Journal of Allergy and Clinical Immunology·J Day
Sep 12, 1998·Primary Care·D Weldon
Nov 18, 2000·The Journal of Allergy and Clinical Immunology·G D Marshall
Nov 15, 2001·The Journal of Allergy and Clinical Immunology·J BousquetUNKNOWN World Health Organization

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.