Antibacterial Activity and Antibiotic-Enhancing Effects of Honeybee Venom against Methicillin-Resistant Staphylococcus aureus

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Sangmi HanSok Cheon Pak

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA), along with other antibiotic resistant bacteria, has become a significant social and clinical problem. There is thus an urgent need to develop naturally bioactive compounds as alternatives to the few antibiotics that remain effective. Here we assessed the in vitro activities of bee venom (BV), alone or in combination with ampicillin, penicillin, gentamicin or vancomycin, on growth of MRSA strains. The antimicrobial activity of BV against MRSA strains was investigated using minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC) and a time-kill assay. Expression of atl which encodes murein hydrolase, a peptidoglycan-degrading enzyme involved in cell separation, was measured by reverse transcription-polymerase chain reaction. The MICs of BV were 0.085 µg/mL and 0.11 µg/mL against MRSA CCARM 3366 and MRSA CCARM 3708, respectively. The MBC of BV against MRSA 3366 was 0.106 µg/mL and that against MRSA 3708 was 0.14 µg/mL. The bactericidal activity of BV corresponded to a decrease of at least 3 log CFU/g cells. The combination of BV with ampicillin or penicillin yielded an inhibitory concentration index ranging from 0.631 to 1.002, indicating a partial and ...Continue Reading

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Citations

Nov 12, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Woon-Hae KimKwan-Kyu Park
Feb 20, 2018·Applied Microbiology and Biotechnology·Lasse van GeelenRainer Kalscheuer
Oct 4, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Reza AkbariKamran Pooshang Bagheri
Jul 16, 2020·Toxins·Hesham El-SeediShaden Khalifa
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Irina TanuwidjajaMirna Mrkonjić Fuka
Jul 3, 2021·Antibiotics·Rita Abou NaderZiad Fajloun
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Magdalena RatajczakJan Matysiak

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Methods Mentioned

BETA
size exclusion gel chromatography
PCR
electrophoresis

Software Mentioned

SPSS
BLAST

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