Antibacterial activity and immunomodulatory effects on a bovine mammary epithelial cell line exerted by nisin A-producing Lactococcus lactis strains

Journal of Dairy Science
M MalvisiR Piccinini

Abstract

Twenty-nine strains of mastitis pathogens were used to study the antibacterial activity of the cell-free supernatants (CFS) of 25 strains of Lactococcus lactis ssp. lactis. Out of the tested strains, only the CFS of L. lactis LL11 and SL153 were active, inhibiting and killing most of the pathogens. By means of ultra-performance liquid chromatography/high resolution mass spectrometry, they were shown to produce nisin A, a class I bacteriocin. A variable sensitivity to nisin A-containing CFS was observed among Streptococcus uberis and Enterococcus faecalis strains. Nonetheless, Streptococcus agalactiae, Strep. uberis, and E. faecalis displayed high minimum inhibitory concentration values, reaching 384 arbitrary units/mL. Interestingly, the minimum inhibitory values and the bactericidal concentrations were almost identical among them for each of the 2 stains, LL11 and SL153. Staphylococci were, on average, less sensitive than streptococci, but the 2 CFS inhibited and killed, at different dilutions, strains of methicillin-resistant Staphylococcus aureus. The immune response to nisin A-containing CFS was tested using the bovine mammary epithelial cell line BME-UV1. Application of CFS did not damage epithelial integrity, as demonstra...Continue Reading

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Citations

May 23, 2012·Domestic Animal Endocrinology·R J CollierN D Horseman
May 1, 2017·Journal of Dairy Science·M A CrookendenM D Mitchell
Oct 27, 2018·Frontiers in Veterinary Science·Pascal Rainard, Gilles Foucras
Mar 28, 2019·Journal of Animal Physiology and Animal Nutrition·Joanna MałaczewskaAndrzej K Siwicki
Aug 14, 2019·Critical Reviews in Microbiology·Angeliki AngelopoulouR Paul Ross
Dec 29, 2020·Peptides·Joanna Małaczewska, Edyta Kaczorek-Łukowska
May 1, 2021·Animals : an Open Access Journal From MDPI·Juan Carlos Hernández-GonzálezNayeli Shantal Castrejón-Jiménez
May 4, 2021·Oxidative Medicine and Cellular Longevity·Fuqing HuangJin Zhong

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