Antibacterial Activity of Bacteriocinogenic Commensal Escherichia coli against Zoonotic Strains Resistant and Sensitive to Antibiotics

Antibiotics
Justyna Mazurek-PopczykKatarzyna Baldy-Chudzik

Abstract

Antibiotic resistance concerns various areas with high consumption of antibiotics, including husbandry. Resistant strains are transmitted to humans from livestock and agricultural products via the food chain and may pose a health risk. The commensal microbiota protects against the invasion of environmental strains by secretion of bacteriocins, among other mechanisms. The present study aims to characterize the bactericidal potential of bacteriocinogenic Escherichia coli from healthy humans against multidrug-resistant and antibiotic-sensitive strains from pigs and cattle. Bacteriocin production was tested by the double-layer plate method, and bacteriocin genes were identified by the PCR method. At least one bacteriocinogenic E. coli was detected in the fecal samples of 55% of tested individuals, adults and children. Among all isolates (n = 210), 37.1% were bacteriocinogenic and contained genes of colicin (Col) Ib, ColE1, microcin (Mcc) H47, ColIa, ColM, MccV, ColK, ColB, and single ColE2 and ColE7. Twenty-five E. coli carrying various sets of bacteriocin genes were further characterized and tested for their activity against zoonotic strains (n = 60). Strains with ColE7 (88%), ColE1-ColIa-ColK-MccH47 (85%), MccH47-MccV (85%), ColE...Continue Reading

References

Oct 9, 2001·Trends in Biochemical Sciences·C Kleanthous, D Walker
Jan 23, 2002·Journal of Basic Microbiology·J Smarda, V Obdrzálek
Jun 1, 2002·International Journal of Food Microbiology·Rachel GormanCatherine C Adley
Jan 27, 2004·Antimicrobial Agents and Chemotherapy·Anne-Marie PonsGilles Cottenceau
Jul 15, 2004·Advances in Applied Microbiology·Osnat GillorMargaret A Riley
Oct 7, 2004·Applied and Environmental Microbiology·Gerry P SchambergerFrancisco Diez-Gonzalez
Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
Jun 1, 2007·Journal of Food Protection·Brenda S PattonChad H Stahl
Jul 24, 2007·Journal of Bacteriology·Anne Jeziorowski, David M Gordon
Nov 3, 2010·Current Opinion in Microbiology·Bärbel Stecher, Wolf-Dietrich Hardt
Dec 15, 2010·Journal of Bacteriology·Stephanie Helbig, Volkmar Braun
Mar 3, 2011·FEMS Immunology and Medical Microbiology·Louise BélangerCharles M Dozois
Feb 7, 2012·Bioorganic & Medicinal Chemistry·Rania SoudyKamaljit Kaur
Apr 24, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C EwersL H Wieler
Jun 16, 2012·Nature·UNKNOWN Human Microbiome Project Consortium
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yong-Guan ZhuJames M Tiedje
Oct 8, 2013·Nature Reviews. Immunology·Charlie G Buffie, Eric G Pamer
Mar 19, 2014·The Journal of Antimicrobial Chemotherapy·Aman Preet SinghPraveen Rishi
Apr 28, 2013·Journal of Public Health Research·Stephanie M FletcherJohn T Ellis
Feb 19, 2015·International Journal of Environmental Research and Public Health·Justyna MazurekKatarzyna Baldy-Chudzik
Mar 26, 2015·ELife·Zachary D Blount
Mar 27, 2015·Postȩpy higieny i medycyny doświadczalnej·Katarzyna Baldy-ChudzikJustyna Mazurek
Nov 7, 2015·Microbiology Spectrum·Amee R Manges, James R Johnson
Dec 19, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Amee R Manges
Nov 1, 2016·Nature·Martina Sassone-CorsiManuela Raffatellu
Apr 7, 2017·Journal of Applied Microbiology·J JangS Ishii
Apr 13, 2017·Veterinary Research·Amel Ben LaghaDaniel Grenier

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.