Antibacterial activity of calcium hydroxide combined with chitosan solutions and the outcomes on the bond strength of RealSeal sealer to radicular dentin

Journal of Biomedical Research
Shaymaa E Elsaka, Amr Mohamed Elnaghy


The purpose of this study was to investigate the antibacterial activity of calcium hydroxide [Ca(OH)2] combined with chitosan solutions against Enterococcus faecalis-infected root canal dentin and the effect of this new intracanal medicament on the bond strength of RealSeal sealer to radicular dentin. An experimental intracanal medicament was prepared by mixing different concentrations of chitosan solution (25%, 50%, and 100%, W/V) to Ca(OH)2 powder. Antibacterial activity was evaluated and the total numbers of colony forming units were determined. Bonding ability of RealSeal sealer to radicular dentin was evaluated using push-out bond strength test. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey's multiple comparison tests. We found that Ca(OH)2 combined with different concentrations of chitosan solutions showed better antibacterial activity than Ca(OH)2 mixed with saline, without significantly affecting the bond strength of RealSeal sealer to radicular dentin (P > 0.05). The findings suggest that Ca(OH)2 combined with chitosan is a promising intracanal medicament and may be effective in endodontic therapy.


Nov 7, 1999·International Endodontic Journal·José F Siqueira, Hélio P Lopes
Mar 7, 2002·Microbes and Infection·Brett D Shepard, Michael S Gilmore
Nov 14, 2006·Dental Materials : Official Publication of the Academy of Dental Materials·Denise F S PetriJorge A J S Bocangel
Jul 20, 2007·Journal of Oral Science·Wen L ChaiMariam Abdullah


Feb 8, 2013·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Shaymaa E Elsaka
Jan 18, 2017·Journal of Dental Research, Dental Clinics, Dental Prospects·Ali KangarlouOmid Dianat
Feb 11, 2020·Dental Materials Journal·Apimon SupotngarmkulOranart Matangkasombut
Jan 21, 2021·Journal of Materials Science. Materials in Medicine·José Caetano de SouzaSilmara Aparecida Milori Corona

Related Concepts

Calcium hydroxide
Laboratory Procedures
Enterococcus faecalis
Pulp Canals
Anti-Bacterial Agents
Bactericidal Activity
Endodontic Procedure

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