Antibacterial activity of doxycycline-loaded nanoparticles

Methods in Enzymology
Ranjita Misra, Sanjeeb Kumar Sahoo

Abstract

Doxycycline is a tetracycline antibiotic with a potent antibacterial activity against a wide variety of bacteria. However, poor cellular penetration limits its use for the treatment of infectious disease caused by intracellular pathogens. One potential strategy to overcome this problem is the use of nanotechnology that can help to easily target the intracellular sites of infection. The antibacterial activity of these antibiotics is enhanced by encapsulating it in polymeric nanoparticles. In this study, we describe the improvement of the entrapment efficiency of doxycycline hydrochloride (doxycycline)-loaded PLGA:PCL nanoparticles up to 70% with variation of different formulation parameters such as polymer ratio, amount of drug loading (w/w), solvent selection, electrolyte addition, and pH alteration in the formulation. We have evaluated the efficacy of these nanoparticles over native doxycycline against a strain of Escherichia coli (DH5α) through growth inhibition and colony counting. The results indicate that doxycycline-loaded nanoparticles have superior effectiveness compared to native doxycycline against the above bacterial strain, resulting from the sustained release of doxycycline from nanoparticles. These results are enc...Continue Reading

Citations

Dec 3, 2016·Drug Development and Industrial Pharmacy·Simona DimchevskaKaterina Goracinova
Dec 30, 2014·Journal of Pharmaceutical Sciences·Rahul S KalhapureThirumala Govender
Jun 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrea Díaz-RoaFelio J Bello
May 22, 2020·The Journal of Pharmacy and Pharmacology·Ayat AbdelkaderMahmoud Elsabahy
Feb 27, 2021·Journal of Dentistry·Manuel ToledanoRaquel Osorio
Apr 13, 2021·Advanced Drug Delivery Reviews·Stine Egebro BirkLine Hagner Nielsen
Sep 29, 2021·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Franziska A SchwartzClaus Moser

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Langmuir : the ACS Journal of Surfaces and Colloids
Soracha ThamphiwatanaLiangfang Zhang
Indian Journal of Biochemistry & Biophysics
Radhika Ramachandran, Piramanayagam Shanmughavel
© 2021 Meta ULC. All rights reserved