Antibacterial activity of Staphylococcus aureus biofilm under combined exposure of glutaraldehyde, near-infrared light, and 405-nm laser

PloS One
Van Nam TranHyun Wook Kang

Abstract

Healthcare-associated infections have increasingly become problematic in the endoscopic procedures resulting in several severe diseases such as carbapenem-resistant Enterobacteriaceae (CRE)-related infections, pneumonia, and bacteremia. Especially, some bacterial strains are resistant to traditional antimicrobials. Therefore, the necessity of developing new antibiotics or management to deal with bacterial infections has been increasing. The current study combined a low concentration of glutaraldehyde (GTA) with near-infrared (NIR) light and 405-nm laser to entail antibacterial activity on Staphylococcus aureus biofilm. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and colony forming unit (CFU) counting were used to quantify the viable cells while fluorescent and scanning electron microscopic images were used to qualitatively evaluate the cell membrane integrity and structural deformation, respectively. Practically, S. aureus biofilm was highly susceptible (7% cell viability and 6.8-log CFU/cm2 bacterial reduction for MTT assay and CFU analysis, respectively) to the combination of GTA (0.1%), NIR light (270 J/cm2), and 405-nm laser (288 J/cm2) exposure. GTA could form either DNA-protein or protein-prot...Continue Reading

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Citations

Dec 14, 2018·International Journal of Molecular Sciences·Cristina CattòFrancesca Cappitelli
Oct 7, 2020·Critical Reviews in Microbiology·Fazlurrahman KhanYoung-Mog Kim
Mar 4, 2021·Journal of Agricultural and Food Chemistry·Fazlurrahman KhanYoung-Mog Kim

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Methods Mentioned

BETA
chips
irradiate
flow cytometry

Software Mentioned

SPSS

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