Antibacterial and antibiofilm mechanism of eugenol against antibiotic resistance Vibrio parahaemolyticus

Food Microbiology
Md AshrafudoullaSang-Do Ha

Abstract

The objective of this study was to investigate the antibacterial and antibiofilm activity of eugenol against V. parahaemolyticus planktonic and biofilm cells and the involved mechanisms as well. Atime-kill assay, a biofilm formation assay on the surface of crab shells, an assay to determine the reduction of virulence using eugenol at different concentrations, energy-filtered transmission electron microscope (EF-TEM), field emission scanning electron microscopy (FE-SEM), confocal laser scanning microscope (CLSM) and high-performance liquid chromatography (HPLC) were performed to evaluate the antibacterial and antibiofilm activity of eugenol. The results indicated that different concentrations of eugenol (0.1-0.6%) significantly reduced biofilm formation, metabolic activities, and secretion of extracellular polysaccharide (EPS), with effective antibacterial effect. Eugenol at 0.4% effectively eradicated the biofilms formed by clinical and environmental V. parahaemolyticus on crab surface by more than 4.5 and 4 log CFU/cm2, respectively. At 0.6% concentration, the reduction rates of metabolic activities for ATCC27969 and NIFS29 were 79% and 68%, respectively. Whereas, the reduction rates of EPS for ATCC27969 and NIFS29 were 78% an...Continue Reading

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Citations

May 22, 2020·Critical Reviews in Food Science and Nutrition·Md AshrafudoullaSang-Do Ha
Aug 20, 2021·Frontiers in Microbiology·Ezhaveni SathiyamoorthiJintae Lee
Sep 8, 2021·Biotechnology and Applied Biochemistry·Jinhua ZhangSanhong Fan
Jan 12, 2022·Applied Microbiology and Biotechnology·Bingren TianYumei Liu

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