Antibacterial, antifungal, anticancer activities and structural bioinformatics analysis of six naturally occurring temporins

Peptides
Biswajit MishraGuangshun Wang

Abstract

Antimicrobial peptides are a special class of natural products with potential applications as novel therapeutics. This study focuses on six temporins (four with no activity data and two as positive controls). Using synthetic peptides, we report antibacterial, antifungal, and anticancer activities of temporins-CPa, CPb, 1Ga, 1Oc, 1Ola, and 1SPa. While temporin-1Ga and temporin-1OLa showed higher antifungal and anticancer activity, most of these peptides were active primarily against Gram-positive bacteria. Temporin-1OLa, with the highest cell selectivity index, could preferentially kill methicillin-resistant Staphylococcus aureus (MRSA), consistent with a reduced hemolysis in the presence of bacteria. Mechanistically, temporin-1OLa rapidly killed MRSA by damaging bacterial membranes. Using micelles as a membrane-mimetic model, we determined the three-dimensional structure of temporin-1OLa by NMR spectroscopy. The peptide adopted a two-domain structure where a hydrophobic patch is followed by a classic amphipathic helix covering residues P3-I12. Such a structure is responsible for anti-biofilm ability in vitro and in vivo protection of wax moths Galleria mellonella from staphylococcal infection. Finally, our bioinformatic analysi...Continue Reading

Citations

Feb 26, 2019·Protein and Peptide Letters·Dan ZhangShufang Liang
Sep 29, 2019·International Journal of Molecular Sciences·Anna GoldaJoanna Koziel
Nov 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhipeng XieYingxia Zhang
Aug 14, 2020·Canadian Journal of Microbiology·Shikha Kapil, Vipasha Sharma
Dec 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hanqi WeiYingxia Zhang
Aug 8, 2021·International Journal of Molecular Sciences·Isabella Hernández-Aristizábal, Iván Darío Ocampo-Ibáñez
Sep 17, 2021·Protein Science : a Publication of the Protein Society·Guangshun WangZhe Wang

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