Antibacterial Attributes of Apigenin, Isolated from Portulaca oleracea L

International Journal of Bacteriology
Hanumantappa B NayakaAsha Tukappa

Abstract

The flavonoid apigenin was isolated from aerial part of P. oleracea L. The dried sample of plant was powdered and subjected to soxhlet extractor by adding 80 mL of ethanol : water (70 : 30). The extract was centrifuged at 11000 rpm for 30 min; supernatant was taken for further use. The fraction was concentrated and subjected to PTLC. The R f value of isolated apigenin was calculated (0.82). Purified material was also subjected to its IR spectra, LC-MS, NMR, and HPLC for structural elucidation. The apigenin so-obtained was subjected to antibacterial activity on five pathogenic bacterial strains like Pseudomonas aeruginosa, Salmonella typhimurium, Proteus mirabilis, Klebsiella pneumoniae and Enterobacter aerogenes; among all the bacterial strains, Salmonella typhimurium (17.36 ± 0.18) and Proteus mirabilis (19.12 ± 0.01) have shown maximum diameter of inhibition zone for flavonoid and remaining bacterial strains have shown moderate diameter of inhibition zone when compared with control values 14.56 ± 0.21 and 11.68 ± 0.13, respectively. The minimum inhibitory concentration (MIC) of the flavonoid isolated from P. oleracea L. was tested at the concentration ranging from undiluted sample to 10 mg per mL of concentration. The minimum...Continue Reading

References

Sep 19, 1998·Journal of Ethnopharmacology·I AhmadF Mohammad
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Citations

Aug 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Minqian WangKit Yam
Apr 19, 2018·Natural Product Research·Fen XiuDidier Stien
Jan 7, 2020·Current Drug Metabolism·Pooja Rathaur, Kaid Johar Sr
Apr 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cristina AlcántaraFrancisco J Barba
Jan 19, 2018·FEMS Yeast Research·Heejeong LeeDong Gun Lee
May 31, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Izabela Przybyłek, Tomasz M Karpiński
Apr 10, 2019·Current Medicinal Chemistry·Iolanda Francolini, Antonella Piozzi
May 1, 2021·Natural Product Research·Wen XuDidier Stien
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hlabana Alfred SeepeWinston Nxumalo
Aug 28, 2021·Nutrients·Priyanshu BhargavaSunil C Kaul

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Methods Mentioned

BETA
column chromatography
NMR

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