Antibacterial effects and cytotoxicity of an adhesive containing low concentration of silver nanoparticles

Journal of Dentistry
Maristela Dutra-CorreaIgor S Medeiros

Abstract

To evaluate the antibacterial effects, cytotoxicity and microtensile bond strength of an adhesive containing low concentrations of silver nanoparticles (NAg). Various concentrations of NAg (50, 100, 150, 200 and 250 ppm) were incorporated into the primer of the Scotchbond Multi-Purpose adhesive system (SBMP). Antibacterial activity was examined using a broth microdilution assay to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), agar diffusion assay and the MTT assay was used to examine the biofilm metabolic activity (S. mutans). The Microtensile Bond Test (μTBS) was performed after 24 h, followed by 6-months storage in distilled water. Cytotoxicity was assessed with an MTT reduction assay in human dental pulp stem cells viability after exposure to Nag-conditioned culture media during 0, 24, 48, and 72 h. The results were statistically analyzed (α ≤ 0.05). MIC was found between NAg 25 and 50 ppm MBC was determined at 50 ppm of NAg. Bacterial activity inhibition was higher than control in all NAg groups compared to control in agar diffusion assay. Biofilm inhibition was statistically higher in 250 ppm NAg than control. All NAg groups and SBMP presented similar cytotoxicity in each pe...Continue Reading

Citations

Mar 15, 2019·Dental Materials Journal·Cintia Helena Coury SaraceniIvana Suffredini
Mar 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenjing Song, Shaohua Ge
Jul 11, 2020·Bioengineering·Naji KharoufVincent Ball
Nov 5, 2020·Polymers·Marta Chrószcz, Izabela Barszczewska-Rybarek
Dec 9, 2020·Acta Biomaterialia·Yazi WangX X Zhu
Jan 23, 2021·International Journal of Environmental Research and Public Health·Pune N PaquéThomas Attin
Mar 2, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Zohaib AkramAmr S Fawzy
Jul 25, 2021·The Journal of Prosthetic Dentistry·Gabriela MalateauxLuciano Lauria Dib
Jul 24, 2021·International Journal of Nanomedicine·Juanjuan XuGaofeng Liang

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