Antibacterial Effects of Bicarbonate in Media Modified to Mimic Cystic Fibrosis Sputum.

International Journal of Molecular Sciences
Pongsiri JaikumpunAkos Zsembery

Abstract

Cystic fibrosis (CF) is a hereditary disease caused by mutations in the gene encoding an epithelial anion channel. In CF, Cl- and HCO3- hyposecretion, together with mucin hypersecretion, leads to airway dehydration and production of viscous mucus. This habitat is ideal for colonization by pathogenic bacteria. We have recently demonstrated that HCO3- inhibits the growth and biofilm formation of Pseudomonas aeruginosa and Staphylococcus aureus when tested in laboratory culture media. Using the same bacteria our aim was to investigate the effects of HCO3- in artificial sputum medium (ASM), whose composition resembles CF mucus. Control ASM containing no NaHCO3 was incubated in ambient air (pH 7.4 or 8.0). ASM containing NaHCO3 (25 and 100 mM) was incubated in 5% CO2 (pH 7.4 and 8.0, respectively). Viable P. aeruginosa and S. aureus cells were counted by colony-forming unit assay and flow cytometry after 6 h and 17 h of incubation. Biofilm formation was assessed after 48 h. The data show that HCO3- significantly decreased viable cell counts and biofilm formation in a concentration-dependent manner. These effects were due neither to extracellular alkalinization nor to altered osmolarity. These results show that HCO3- exerts direct an...Continue Reading

References

Jan 22, 1998·Canadian Journal of Microbiology·M Ghani, J S Soothill
Apr 5, 2002·Clinical Microbiology Reviews·Jeffrey B LyczakGerald B Pier
Nov 30, 2002·Advanced Drug Delivery Reviews·R C Boucher
Nov 11, 2003·Journal of Microbiological Methods·Daniel HoefelChristopher P Saint
Jun 11, 2005·Journal of Medical Microbiology·Dinesh D SriramuluUte Römling
Jul 6, 2006·Applied and Environmental Microbiology·H-L AlakomiM Saarela
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hirotoshi MatsuiRichard C Boucher
Sep 18, 2007·Journal of Bacteriology·Kelli L PalmerMarvin Whiteley
Oct 31, 2007·Antimicrobial Agents and Chemotherapy·Mrinalini SharmaPietro Speziale
Sep 4, 2009·The Journal of Clinical Investigation·Mary Abigail S GarciaPaul M Quinton
Mar 26, 2010·Folia Microbiologica·A HostackáM Stefkovicová
May 3, 2012·MBio·Amy T Y YeungRobert E W Hancock
May 31, 2012·TheScientificWorldJournal·Emer P ReevesNoel G McElvaney
Jan 29, 2013·Applied and Environmental Microbiology·Volker BehrendsJacob G Bundy
Feb 5, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Alessandra Livraghi-ButricoBarbara R Grubb
Jul 17, 2014·American Journal of Respiratory and Critical Care Medicine·Susan E BirketSteven M Rowe
Oct 16, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Kieth J Thomas, Charles V Rice
Dec 10, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Luke A CliftonJeremy H Lakey
Jul 15, 2015·Advances in Wound Care·Simon Finnegan, Steven L Percival
Jan 7, 2017·BMC Microbiology·Emily V DaviesCraig Winstanley
Jul 25, 2017·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Karl KunzelmannHans Beat Hadorn
Jan 13, 2018·JCI Insight·Susan E BirketSteven M Rowe
Oct 5, 2018·Frontiers in Microbiology·Orsolya DobayÁkos Zsembery
Nov 16, 2018·Annals of the American Thoracic Society·Anna ErmundGunnar C Hansson
Apr 26, 2019·Scientific Reports·Juliette SimoninIsabelle Sermet-Gaudelus

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

Flowing
GraphPad
BD CellQuest Pro
GraphPad Prism
ImageJ

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