Antibacterial Effects of Phage Lysin LysGH15 on Planktonic Cells and Biofilms of Diverse Staphylococci

Applied and Environmental Microbiology
Yufeng ZhangJingmin Gu

Abstract

Treatment of infections caused by staphylococci has become more difficult because of the emergence of multidrug-resistant strains as well as biofilm formation. In this study, we observed the ability of the phage lysin LysGH15 to eliminate staphylococcal planktonic cells and biofilms formed by Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus haemolyticus, and Staphylococcus hominis All these strains were sensitive to LysGH15, showing reductions in bacterial counts of approximately 4 log units within 30 min after treatment with 20 μg/ml of LysGH15, and the MICs ranged from 8 μg/ml to 32 μg/ml. LysGH15 efficiently prevented biofilm formation by the four staphylococcal species at a dose of 50 μg/ml. At a higher dose (100 μg/ml), LysGH15 also showed notable disrupting activity against 24-h and 72-h biofilms formed by S. aureus and coagulase-negative species. In the in vivo experiments, a single intraperitoneal injection of LysGH15 (20 μg/mouse) administered 1 h after the injection of S. epidermidis at double the minimum lethal dose was sufficient to protect the mice. The S. epidermidis cell counts were 4 log units lower in the blood and 3 log units lower in the organs of mice 24 h after treatment with LysGH15 than i...Continue Reading

References

May 4, 1989·The New England Journal of Medicine·W Brumfitt, J Hamilton-Miller
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Jan 11, 2000·Clinical Microbiology Reviews·M M DingesP M Schlievert
Mar 4, 2000·Journal of Microbiological Methods·S StepanovicM Svabic-Vlahovic
Nov 14, 2003·The Journal of Antimicrobial Chemotherapy·Isabel JadoUNKNOWN Spanish Pneumococcal Infection Study Network
Jul 11, 2006·International Journal of Antimicrobial Agents·Wilma ZiebuhrSvetlana Kozitskaya
Nov 7, 2006·Applied and Environmental Microbiology·Peter Sass, Gabriele Bierbaum
Oct 31, 2007·Antimicrobial Agents and Chemotherapy·Mrinalini SharmaPietro Speziale
Feb 21, 2008·Antimicrobial Agents and Chemotherapy·Rawya S Al-Dhaheri, L Julia Douglas
Oct 1, 2008·Current Opinion in Microbiology·Vincent A Fischetti
Jan 13, 2009·Infectious Disease Clinics of North America·Kathie L RogersMark E Rupp
Jul 22, 2009·Antimicrobial Agents and Chemotherapy·Richard KuehlRegine Landmann
Sep 15, 2009·Current Medicinal Chemistry·E A LarkinB G Stiles
Jul 31, 2012·Journal of Virology·Jingmin GuWenyu Han
Aug 29, 2012·Antimicrobial Agents and Chemotherapy·Jongkon SaisingFriedrich Götz
Sep 10, 2014·PloS One·Diana GutiérrezPilar García
Jan 24, 2015·International Journal of Antimicrobial Agents·J ClaessensJ Van Eldere
Jan 30, 2015·The Journal of Antimicrobial Chemotherapy·Mathias SchmelcherDavid M Donovan
Aug 9, 2016·Applied Microbiology and Biotechnology·Yoonjee Chang, Sangryeol Ryu
Mar 28, 2017·Applied Microbiology and Biotechnology·Murugesan SivaranjaniArumugam Veera Ravi
May 4, 2017·Antimicrobial Agents and Chemotherapy·Raymond SchuchMichael Wittekind
Jun 1, 2017·Antimicrobial Agents and Chemotherapy·Nethravathi PoonachaUmender Sharma
Aug 3, 2017·Nutrition Research and Practice·Eunkyo ParkJung-Hyun Kim
Nov 29, 2017·Antimicrobial Agents and Chemotherapy·Shankaramurthy ChannabasappaBharathi Sriram

❮ Previous
Next ❯

Citations

Nov 22, 2018·Frontiers in Immunology·Roberto VázquezPedro García
Sep 19, 2020·Applied Microbiology and Biotechnology·Amala BhagwatJonathan S Dordick
Aug 15, 2020·Microbiology and Molecular Biology Reviews : MMBR·Katrin Schilcher, Alexander R Horswill
Dec 29, 2020·Critical Reviews in Microbiology·Wen YinJin He
Feb 10, 2021·Pathogens·Angela FrançaLuís D R Melo
May 4, 2021·Frontiers in Cellular and Infection Microbiology·Yifei LuShuguang Lu
Nov 13, 2019·International Journal of Biological Macromolecules·Ramachandran SrinivasanKodiveri Muthukaliannan Gothandam

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.