Abstract
Porphyromonas species are closely associated with companion animal periodontitis which is one of the most common diseases in dogs and cats and leads to serious systemic diseases if left untreated. In this study, we evaluated the antimicrobial effects and mode of action of sodium tripolyphosphate (polyP3, Na₅P₃O10), a food additive with proven safety, using three pathogenic Porphyromonas species. The minimum inhibitory concentrations (MICs) of polyP3 against Porphyromonas gulae, Porphyromonas cansulci, and Porphyromonas cangingivalis were between 500 and 750 mg/L. PolyP3 significantly decreased viable planktonic cells as well as bacterial biofilm formation, even at sub-MIC concentrations. PolyP3 caused bacterial membrane disruption and this effect was most prominent in P. cangingivalis, which was demonstrated by measuring the amount of nucleotide leakage from the cells. To further investigate the mode of action of polyP3, high-throughput whole-transcriptome sequencing was performed using P. gulae. Approximately 30% of the total genes of P. gulae were differentially expressed by polyP3 (> 4-fold, adjusted p value < 0.01). PolyP3 influenced the expression of the P. gulae genes related to the biosynthesis of thiamine, ubiquinone, a...Continue Reading
References
Dec 1, 1991·Biochimie·R Mikkola, C G Kurland
Jan 1, 1972·Die Nahrung·I F Ryabinin
Oct 1, 1994·International Journal of Systematic Bacteriology·M D CollinsD I Wigney
Feb 8, 1997·The Veterinary Record·R P AllakerJ M Hardie
Mar 12, 1998·Journal of Clinical Periodontology·S S SocranskyR L Kent
Oct 21, 1998·The Veterinary Clinics of North America. Small Animal Practice·C E Harvey
Jun 29, 2000·Annual Review of Biochemistry·A KornbergD Ault-Riché
Oct 18, 2000·Journal of Clinical Periodontology·L A Ximénez-FyvieS S Socransky
Jun 20, 2001·International Journal of Systematic and Evolutionary Microbiology·D FournierC Ménard
May 16, 2002·Periodontology 2000·Jørgen Slots, Michael G Jorgensen
Nov 1, 2002·Nature·Wade WinklerRonald R Breaker
Dec 18, 2002·Journal of Bacteriology·Wai-Leung NgMalcolm E Winkler
Jul 1, 1956·Journal of General Microbiology·C S CUMMINS, H HARRIS
Feb 20, 2004·Annals of Periodontology·Frank A ScannapiecoSusanna Paju
Mar 5, 2004·Oral Diseases·K OkudaK Ishihara
May 27, 2006·Nature·Alexander SerganovDinshaw J Patel
Oct 20, 2006·Pathologie-biologie·C BodetD Grenier
Dec 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Maja RaschkeTeresa B Fitzpatrick
Apr 16, 2008·Antimicrobial Agents and Chemotherapy·Bernd StephanPeter Silley
May 16, 2008·Journal of Medical Microbiology·Jenny E EmersonNeil F Fairweather
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Bruce A Maguire
Nov 26, 2010·Antimicrobial Agents and Chemotherapy·Ji-Hoi MoonJin-Yong Lee
Jan 15, 2011·International Journal of Biological Sciences·Qinglin DuJianping Xie
Jul 10, 2012·Journal of Dental Research·R P DarveauM A Curtis
Dec 19, 2013·PloS One·Ian J DavisStephen Harris
Aug 26, 2014·BMC Microbiology·Ji-Hoi MoonJin-Yong Lee
May 4, 2015·The Journal of Microbiology·Ji-Hoi MoonJin-Yong Lee
Nov 26, 2015·Antimicrobial Agents and Chemotherapy·Eun-Young JangJin-Yong Lee
Dec 18, 2015·Nucleic Acids Research·Robert D FinnAlex Bateman
May 1, 1991·Journal of Food Protection·S J KnabelP A Hartman
Apr 1, 1994·Journal of Food Protection·Ruby M LeeFred D Williams