Antibacterial effects of sodium tripolyphosphate against Porphyromonas species associated with periodontitis of companion animals

Journal of Veterinary Science
Jae Hyung LeeJin Yong Lee

Abstract

Porphyromonas species are closely associated with companion animal periodontitis which is one of the most common diseases in dogs and cats and leads to serious systemic diseases if left untreated. In this study, we evaluated the antimicrobial effects and mode of action of sodium tripolyphosphate (polyP3, Na₅P₃O10), a food additive with proven safety, using three pathogenic Porphyromonas species. The minimum inhibitory concentrations (MICs) of polyP3 against Porphyromonas gulae, Porphyromonas cansulci, and Porphyromonas cangingivalis were between 500 and 750 mg/L. PolyP3 significantly decreased viable planktonic cells as well as bacterial biofilm formation, even at sub-MIC concentrations. PolyP3 caused bacterial membrane disruption and this effect was most prominent in P. cangingivalis, which was demonstrated by measuring the amount of nucleotide leakage from the cells. To further investigate the mode of action of polyP3, high-throughput whole-transcriptome sequencing was performed using P. gulae. Approximately 30% of the total genes of P. gulae were differentially expressed by polyP3 (> 4-fold, adjusted p value < 0.01). PolyP3 influenced the expression of the P. gulae genes related to the biosynthesis of thiamine, ubiquinone, a...Continue Reading

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Citations

Jan 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiangju SunYuhua Fan

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Datasets Mentioned

BETA
GSE93901

Methods Mentioned

BETA
scanning electron microscopy
RNA-Seq

Software Mentioned

ImageJ
eggNOG
- mapper
DESeq2 R
GraphPad
GraphPad Prism
Bowtie2

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