Antibacterial Monoclonal Antibodies Do Not Disrupt the Intestinal Microbiome or Its Function.

Antimicrobial Agents and Chemotherapy
Omari Jones-NelsonBret R Sellman

Abstract

Antibiotics revolutionized the treatment of infectious diseases; however, it is now clear that broad-spectrum antibiotics alter the composition and function of the host's microbiome. The microbiome plays a key role in human health, and its perturbation is increasingly recognized as contributing to many human diseases. Widespread broad-spectrum antibiotic use has also resulted in the emergence of multidrug-resistant pathogens, spurring the development of pathogen-specific strategies such as monoclonal antibodies (MAbs) to combat bacterial infection. Not only are pathogen-specific approaches not expected to induce resistance in nontargeted bacteria, but they are hypothesized to have minimal impact on the gut microbiome. Here, we compare the effects of antibiotics, pathogen-specific MAbs, and their controls (saline or control IgG [c-IgG]) on the gut microbiome of 7-week-old, female, C57BL/6 mice. The magnitude of change in taxonomic abundance, bacterial diversity, and bacterial metabolites, including short-chain fatty acids (SCFA) and bile acids in the fecal pellets from mice treated with pathogen-specific MAbs, was no different from that with animals treated with saline or an IgG control. Conversely, dramatic changes were observe...Continue Reading

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Citations

Apr 13, 2021·ACS Infectious Diseases·Stephen A KellyBrendan F Gilmore

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Datasets Mentioned

BETA
PRJEB34462

Methods Mentioned

BETA
PCR
feature extraction

Software Mentioned

BBTools
R package vegan
MGSAT
R package Dada2
R
Profinder Archive
Leica Lightning
Agilent Profinder
Airyscan
Dada2

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