Antibacterial non-glycosidase activity of invertebrate destabilase-lysozyme and of its helical amphipathic peptides

Chemotherapy
L ZavalovaI Baskova

Abstract

Since bactericidal properties of some lysozymes are independent of their glycosidase activity, we have investigated this phenomenon for destabilase-lysozyme (DL) from medicinal leech (Hirudo medicinalis). Glycosidase activity was determined on Micrococcus luteus, non-enzymatic antibacterial activity of heat-treated DL and of synthetic peptides alpha1, alpha2 and alpha3 (fragments of its primary structure) on M. luteus, Escherichia coli, Bacillus brevis and Streptomyces chrysomallus. Glycosidase activity disappeared after the heating of native DL at 100 degrees C for 40 min. Antibacterial activity of heat-treated DL for M. luteus MDMSU128 and MDMSU140 expressed as minimal inhibitory concentration was 9.8.10(-8) and 12.10(-8) M, respectively, and to E. coli MDMSU52 11.10(-8) M. Antibacterial activity of synthetic peptide alpha1 for M. luteus MDMSU128 and for E. coli MDMSU52 was 8.3.10(-5) and 4.9.10(-5) M, respectively. DL is the first invertebrate lysozyme with combined enzymatic and non-enzymatic antibacterial action.

Citations

Nov 10, 2011·Die Naturwissenschaften·Jan-Peter Hildebrandt, Sarah Lemke
Apr 24, 2010·Journal of Biosciences·Lien Callewaert, Chris W Michiels
Nov 17, 2010·Biochemistry. Biokhimii︠a︡·L L ZavalovaI P Baskova
Aug 5, 2008·Bioorganicheskaia khimiia·I P Baskova, L L Zavalova
Jan 24, 2009·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Qian RenJinxing Wang
Jun 23, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Naoki Itoh, Keisuke G Takahashi
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May 1, 2020·Biomedicines·Sarah Lemke, Andreas Vilcinskas
Jul 16, 2014·Biomedit︠s︡inskai︠a︡ khimii︠a︡·Iu I FadeevaL L Zavalova
Jul 25, 2019·Fish & Shellfish Immunology·Huiying LiJianmin Yang
Jan 4, 2018·Integrative Medicine Research·Ali K SigErkan Ozmen

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